In vivo study of the initial bacterial adhesion on different implant materials

Abstract Objective Biofilm formation on implant materials plays a major role in the aetiology of periimplantitis. The aim of this study was to examine in vivo the initial bacterial adhesion on six different implant materials. Methods The implant materials Ti-m, TiUnite® , ZiUnite® , ATZ-m, ATZ-s, TZ...

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Bibliographic Details
Published in:Archives of oral biology 2013-09, Vol.58 (9), p.1139-1147
Main Authors: Al-Ahmad, A, Wiedmann-Al-Ahmad, M, Fackler, A, Follo, M, Hellwig, E, Bächle, M, Hannig, C, Han, J.-S, Wolkewitz, M, Kohal, R
Format: Article
Language:English
Subjects:
Adult
Advanced Basic Science
Animals
Bacteria
Bacterial Adhesion - physiology
Bacterial Load - methods
Biofilms - growth & development
Cattle
Dental Alloys - chemistry
Dental Enamel - microbiology
Dental Implants - microbiology
Dentistry
Female
Humans
Implant material
In Situ Hybridization, Fluorescence
Male
Microscopy, Atomic Force
Microscopy, Confocal
Microscopy, Electron, Scanning
Middle Aged
Oral biofilm
Periimplantitis
Streptococcus - growth & development
Surface Properties
Titanium
Titanium - chemistry
Zirconia
Zirconium - chemistry
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Summary:Abstract Objective Biofilm formation on implant materials plays a major role in the aetiology of periimplantitis. The aim of this study was to examine in vivo the initial bacterial adhesion on six different implant materials. Methods The implant materials Ti-m, TiUnite® , ZiUnite® , ATZ-m, ATZ-s, TZP-A-m were tested using bovine enamel slabs as controls. All materials, fixed on splint systems, were examined after 30 min and 120 min of oral exposure. DAPI staining was used for quantitative analysis of the initially adherent microorganisms. Initial adherent microorganisms were visualised by fluorescence In situ -hybridisation (FISH) and quantified by confocal laser scanning microscopy (CLSM). The targets of the oligonucleotide probes were Eubacteria , Veillonella spp., Fusobacterium nucleatum , Actinomyces naeslundii and Streptococcus spp. Results DAPI analysis showed that increasing the time of oral exposure resulted in an increasing amount of initial adherent bacteria. The highest level of colonisation was on ZiUnite® , with the lowest occurring on the bovine enamel, followed by Ti-m. This early colonisation correlated significantly with the surface roughnesses of the materials. FISH and CLSM showed no significant differences relating to total bacterial composition. However, Streptococcus spp. was shown to be the main colonisers on each of the investigated materials. Conclusion it could be shown that within an oral exposure time of 30 min and 120 min, despite the salivary acquired pellicle initial biofilm formation is mainly influenced directly or indirect by the material surface topography. Highly polished surfaces should minimise the risk of biofilm formation, plaque accumulation and possibly periimplantitis.
ISSN:0003-9969
1879-1506
DOI:10.1016/j.archoralbio.2013.04.011