Specific features of Zn2+, Co2+ and Ni2+ ion binding to DNA in alkaline solutions

Dependence of DNA metallization degree during B-DNA transition into the metallized (m) form on DNA concentration has been studied by visible and differential UV-spectroscopy in the presence of Zn2+, Co2+ and Ni2+ ions in tetraborate buffer (pH 8.5) with and without ethidium bromide. Constants of Mt2...

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Bibliographic Details
Published in:International journal of biological macromolecules 2013-04, Vol.55, p.137-141
Main Authors: Sorokin, V.A., Valeev, V.A., Usenko, E.L., Rakovsky, Ya.P., Andrushchenko, V.V.
Format: Article
Language:English
Subjects:
Alkaline solution
B-DNA
Cations - chemistry
cobalt
Cobalt - chemistry
Differential UV spectroscopy
DNA - chemistry
DNA metallization
DNA–metal complex
ethidium
Ethidium - chemistry
Ethidium bromide
heat
Hydrogen-Ion Concentration
ions
m-form
nickel
Nickel - chemistry
Solutions
temperature
ultraviolet-visible spectroscopy
zinc
Zinc - chemistry
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Summary:Dependence of DNA metallization degree during B-DNA transition into the metallized (m) form on DNA concentration has been studied by visible and differential UV-spectroscopy in the presence of Zn2+, Co2+ and Ni2+ ions in tetraborate buffer (pH 8.5) with and without ethidium bromide. Constants of Mt2+ binding to double stranded DNA were calculated. The obtained binding constants corresponded to the formation of inter-strand metal bridges stabilizing m-form. Thermodynamic origin of higher efficiency of Zn2+ ions in DNA metallization compared to Co2+ and Ni2+ was revealed. Increase of the DNA helix-coil transition temperature by up to 10°С upon formation of m-form in the presence of Zn2+ ions was observed and rationalized. Furthermore, a strong cooperative decrease (up to 30°С) of the temperature of В→m transition induced by heating in the presence of Zn2+ was found and its nature was explained.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2013.01.001