Polymer immobilized enzyme optrodes for the detection of penicillin

The preparation and performance of two enzyme-based fiber-optic sensors (optrodes) capable of detecting penicillin are described. Each sensor consists of a polymer membrane covalently attached to the tip of a glass optical fiber. The membrane contains the enzyme penicillinase and a pH-sensitive fluo...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 1987-12, Vol.59 (24), p.2849-2853
Main Authors: Kulp, T J, Camins, I, Angel, S M, Munkholm, C, Walt, DR
Format: Article
Language:English
Subjects:
fiberoptics
fluorescence
immobilized enzymes
penicillin
penicillinase
Online Access:Get full text
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Summary:The preparation and performance of two enzyme-based fiber-optic sensors (optrodes) capable of detecting penicillin are described. Each sensor consists of a polymer membrane covalently attached to the tip of a glass optical fiber. The membrane contains the enzyme penicillinase and a pH-sensitive fluorescent dye. A signal is produced when the enzyme catalyzes the cleavage of the beta -lactam ring of penicillin to produce penicilloic acid and, a pH change. The sensors differ in the way the polymer membrane is constructed and in the type of pH indicator dye used. Both optrodes exhibit response times (40-60 s) lower than those of the corresponding enzyme electrodes (2 min). Each gives a linear response over the concentration range of 0.00025 to 0.01 M penicillin G. The data indicate that these immobilization strategies produce similar results.
ISSN:0003-2700
DOI:10.1021/ac00151a006