Expression analysis of ovostatin 2 reveals its involvement in proliferation, invasion and angiogenesis of cutaneous malignant melanoma

The relationship of ovostatin 2 (OVOS2) expression with the clinicopathological features of cutaneous malignant melanoma (CMM) was investigated to identify OVOS2 expression in cutaneous melanocytic lesions, and to reveal whether OVOS2 has a function in melanoma progression. Eight specimens of CMM an...

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Published in:Journal of dermatology 2013-11, Vol.40 (11), p.901-910
Main Authors: Huang, Ying-Xue, Qi, Jinliang, Wang, Hong-Sheng, Shao, Xue-Bao, Zeng, Xue-Si, Li, A-Mei, Xu, Xiu-Lian, Sun, Jian-Fang
Format: Article
Language:English
Subjects:
Adolescent
Adult
alpha-Macroglobulins - metabolism
Cell Proliferation
Child
cutaneous malignant melanoma
Female
Humans
immunohistochemistry
Male
Melanoma - metabolism
Melanoma - pathology
Middle Aged
Neoplasm Invasiveness
Neovascularization, Pathologic
nevi
ovostatin 2
protease inhibitor
Skin - pathology
Skin Neoplasms - metabolism
Skin Neoplasms - pathology
Young Adult
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Summary:The relationship of ovostatin 2 (OVOS2) expression with the clinicopathological features of cutaneous malignant melanoma (CMM) was investigated to identify OVOS2 expression in cutaneous melanocytic lesions, and to reveal whether OVOS2 has a function in melanoma progression. Eight specimens of CMM and paracancerous tissue were analyzed using real‐time polymerase chain reaction (PCR) and western blot for the mRNA and protein expression of OVOS2, respectively. Immunohistochemical staining was performed on 52 CMM and 62 nevi, followed by clinicopathological significance analysis. The proliferative cells were visualized by staining with Ki‐67 antibody. The intensity of angiogenesis was assessed by staining with vascular endothelial growth factor (VEGF). Real‐time PCR and western blot analyses showed that OVOS2 was significantly upregulated in cutaneous melanoma than in paired normal skins. Immunohistochemistry showed that 86.5% (45/52) of malignant cases showed OVOS2 cytoplasmic expression compared with 29% (18/62) in benign nevi. OVOS2 expression was significantly higher in invasive and metastatic melanoma than in in situ melanoma (P 
ISSN:0385-2407
1346-8138
DOI:10.1111/1346-8138.12294