A photonic crystal biosensor assay for ferritin utilizing iron-oxide nanoparticles

Iron deficiency anemia afflicts 1 in 3 individuals, mostly women and children worldwide. A novel application using iron-oxide nanoparticles (IONPs) and a photonic crystal (PC) optical biosensor as an immunodiagnostic platform for detection of serum ferritin, a biomarker for iron deficiency, is prese...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2014-06, Vol.56, p.320-327
Main Authors: Peterson, Ross D., Cunningham, Brian T., Andrade, Juan E.
Format: Article
Language:English
Subjects:
Antibodies, Immobilized - chemistry
Biological and medical sciences
Biosensing Techniques - instrumentation
Biosensor
Biosensors
Biotechnology
Ferric Compounds - chemistry
Ferritin
Ferritins - blood
Fundamental and applied biological sciences. Psychology
Humans
Iron deficiency
Iron-oxide nanoparticles
Limit of Detection
Methods. Procedures. Technologies
Nanoparticles - chemistry
Photonic crystal
Photons
Various methods and equipments
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Summary:Iron deficiency anemia afflicts 1 in 3 individuals, mostly women and children worldwide. A novel application using iron-oxide nanoparticles (IONPs) and a photonic crystal (PC) optical biosensor as an immunodiagnostic platform for detection of serum ferritin, a biomarker for iron deficiency, is presented. Human liver ferritin (450kDa), clinical serum controls, and three commercially available ferritin ELISA tests were used to evaluate the PC biosensor assay in terms of inter- and intra-assay variability, spike-recovery (%), limit of detection (LOD), and matrix effects on binding. For the PC biosensor, signal response from label-free, sandwich with secondary antibody (pAb), and pAb functionalized with iron-oxide nanoparticles (FpAb) assays were detected using the Biomolecular Interaction Detection (BIND) system. Bland–Altman analysis was used to evaluate agreement between expected values for ferritin in control sera and each of the detection platforms. Inter- and intra-assay variability of the PC biosensor were both
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2014.01.022