Effect of poly(vinyl acetate–acrylamide) microspheres properties and steric hindrance on the immobilization of Candida rugosa lipase

► Carrier hydrophobicity/hydrophilicity could affect lipase immobilization. ► To enhance lipase activity, lipase/carrier should be rationally designed. ► The optimal immobilized lipase held higher specific activity than free lipase. Poly(vinyl acetate–acrylamide) microspheres were synthesized in the...

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Bibliographic Details
Published in:Bioresource technology 2012-11, Vol.124, p.233-236
Main Authors: Zhang, Dong-Hao, Yuwen, Li-Xia, Li, Chao, Li, Ya-Qiong
Format: Article
Language:English
Subjects:
Acrylamide - chemistry
Candida - enzymology
Candida rugosa
Enzymes, Immobilized - metabolism
Hydrophobic/hydrophilic
Immobilized enzyme
Lipase - metabolism
Microspheres
Polyvinyls - chemistry
Specific activity
Spectroscopy, Fourier Transform Infrared
Steric hindrance
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Summary:► Carrier hydrophobicity/hydrophilicity could affect lipase immobilization. ► To enhance lipase activity, lipase/carrier should be rationally designed. ► The optimal immobilized lipase held higher specific activity than free lipase. Poly(vinyl acetate–acrylamide) microspheres were synthesized in the absence or presence of isooctane via suspension polymerization and utilized as carriers to immobilize Candida rugosa lipase. When the hydrophobic/hydrophilic surface characteristics of the microspheres were modified by changing the ratio of vinyl acetate (hydrophobic monomer) to acrylamide (hydrophilic monomer) from 50:50 to 86:24, the immobilization ratio changed from 45% to 92% and the activity of the immobilized lipase increased from 202.5 to 598.0U/g microsphere. Excessive lipase loading caused intermolecular steric hindrance, which resulted in a decline in lipase activity. The maximum specific activity of the immobilized lipase (4.65U/mg lipase) was higher than that of free lipase (3.00U/mg lipase), indicating a high activity recovery during immobilization.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2012.08.083