Alanyl-glutamine administration suppresses Th17 and reduces inflammatory reaction in dextran sulfate sodium-induced acute colitis

T helper (Th) cells play a major role in the pathogenesis of inflammatory bowel disease (IBD). Glutamine (Gln) is known to have immunomodulatory effects in metabolic stressed conditions. This study investigated the effects of post-treatment of alanyl-glutamine (Ala-Gln) on Th cell-associated cytokin...

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Published in:International immunopharmacology 2013-09, Vol.17 (1), p.1-8
Main Authors: Hou, Yu-Chen, Liu, Jun-Jen, Pai, Man-Hui, Tsou, Shung-Sheng, Yeh, Sung-Ling
Format: Article
Language:English
Subjects:
Alanyl-glutamine
Animals
Body Weight
Colitis - chemically induced
Colitis - drug therapy
Colon
Cytokines - genetics
Cytokines - metabolism
Dextran Sulfate - toxicity
Dipeptides - therapeutic use
DSS-colitis
Gene Expression Regulation - drug effects
Immunoglobin G
Inflammation - chemically induced
Inflammation - drug therapy
Interleukin-17
Male
Mice
Mice, Inbred C57BL
RNA, Messenger - genetics
RNA, Messenger - metabolism
T helper cells
Th17 Cells - drug effects
Th17 Cells - physiology
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Summary:T helper (Th) cells play a major role in the pathogenesis of inflammatory bowel disease (IBD). Glutamine (Gln) is known to have immunomodulatory effects in metabolic stressed conditions. This study investigated the effects of post-treatment of alanyl-glutamine (Ala-Gln) on Th cell-associated cytokine expressions and inflammatory reaction in dextran sulfate sodium (DSS)-induced colitis. C57BL/6 mice received distilled water containing 3% DSS for 5days to induce colitis, whereas the normal control (NC) group received distilled water. After induction of colitis, one of the colitis groups (DG) was intraperitoneally injected with an Ala-Gln solution (0.5gGln/kg/d), and the saline DSS group (DS) received an identical volume of saline. After treatment for 3days, mice were sacrificed, and the blood and tissue samples were collected for further analysis. DSS colitis resulted in higher percentages of blood interleukin (IL)-17-secreting Th cells and greater expression of Th cell-associated cytokine messenger RNA (mRNA) in the mesenteric lymph nodes (MLN). Also, luminal immunoglobin (Ig) G, keratinocyte-derived chemokine, and macrophage chemoattractant protein-1 levels were higher in the DS group than the NC group, whereas these parameters did not differ between the DG and NC groups. The DG group had lower blood IL-17A, 17F, MLN IL-17 mRNA and macrophage percentage in the peritoneal lavage fluid than those of the DS group. These results suggest that post-treatment with Ala-Gln suppressed Th17-associated cytokine expressions, reduced macrophage infiltration into the peritoneal cavity and decreased pro-inflammatory cytokine production in the colon, thus may have attenuated inflammatory response in DSS-induced colitis. •Post-treatment with Ala-Gln attenuated inflammatory response in a model of colitis.•Ala-Gln administration suppressed Th17-associated cytokine production.•Ala-Gln reduced macrophage infiltration and pro-inflammatory cytokine production.
ISSN:1567-5769
1878-1705
DOI:10.1016/j.intimp.2013.05.004