Identification of proteins related to early changes observed in Human hepatocellular carcinoma cells after treatment with the mycotoxin Zearalenone

Zearalenone (ZEA) is a mycotoxin produced by some Fusarium species. ZEA often occur as a contaminant in cereal grains and animal feeds. Human exposure occurs by ingestion of mycotoxin-contaminated products and can cause serious health problems. It was established that this mycotoxin have an hepato,...

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Bibliographic Details
Published in:Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie 2013-09, Vol.65 (6), p.809-816
Main Authors: Gazzah, Amel Chatti, Camoin, Luc, Abid, Salwa, Bouaziz, Chayma, Ladjimi, Moncef, Bacha, Hassen
Format: Article
Language:English
Subjects:
Apoptosis - drug effects
Caspase 3 - metabolism
Cell Culture Techniques
Cell Survival - drug effects
Chromatography, Reverse-Phase
Estrogens, Non-Steroidal - toxicity
feeds
Flow Cytometry
Fusarium
genotoxicity
Hep G2 Cells
Hepatocarcinoma cells (HepG2)
hepatoma
human cell lines
Humans
ingestion
Isobaric tagging for relative and absolute quantification (iTRAQ)
Protein Biosynthesis - drug effects
protein synthesis
proteins
proteomics
Proteomics - methods
small cereal grains
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Time Factors
zearalenone
Zearalenone (ZEA)
Zearalenone - toxicity
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Summary:Zearalenone (ZEA) is a mycotoxin produced by some Fusarium species. ZEA often occur as a contaminant in cereal grains and animal feeds. Human exposure occurs by ingestion of mycotoxin-contaminated products and can cause serious health problems. It was established that this mycotoxin have an hepato, haemato, immuno and genotoxic properties (Maaroufi et al., 1996; Lioi et al., 2004). While most ZEA toxic effects have been quite well investigated, more studies are required to elucidate its mechanisms of toxicity. In order to better understand the molecular mechanisms involved in ZEA toxicity, we used a proteomic approach, to assess the early changes in protein expression initiated by ZEA in HepG2 cells. Our results showed that, after 8h of exposure, cells were still viable and showed a significant change in a number of proteins involved in diverse cellular processes. These changes may provide the early affected functions and yield further insight into mechanisms underlying the involvement of mycotoxin-induced diseases.
ISSN:0940-2993
1618-1433
DOI:10.1016/j.etp.2012.11.007