Strain differences in pleural mesothelial cell reactions induced by potassium octatitanate fibers (TISMO) infused directly into the thoracic cavity

Although we have previously reported that the fiber-shaped TISMO, morphologically similar to asbestos, can induce a severe mesothelial reaction in A/J mice, it is important to clarify any strain differences. In the present study, female A/J, C3H/HeN, ICR and C57BL/6 mice were therefore employed as t...

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Bibliographic Details
Published in:Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie 2013-09, Vol.65 (6), p.925-932
Main Authors: Yokohira, Masanao, Nakano, Yuko, Yamakawa, Keiko, Kishi, Sosuke, Ninomiya, Fumiko, Saoo, Kousuke, Imaida, Katsumi
Format: Article
Language:English
Subjects:
animal models
Animals
asbestos
Body Weight - drug effects
Epithelium - drug effects
Epithelium - pathology
Female
histopathology
humans
Immunohistochemistry
Infusion Pumps
iron
Kidney - drug effects
Kidney - pathology
liver
Liver - drug effects
Liver - pathology
Lung
Lung - drug effects
Lung - pathology
Mesothelioma
Mice
Mouse
Organ Size - drug effects
Particle Size
pleura
Pleural Cavity - drug effects
Pleural Cavity - pathology
potassium
risk
Species Specificity
Strain difference
strain differences
thoracic cavity
thorax
TiO2
TISMO
Titanium - chemistry
Titanium - toxicity
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Summary:Although we have previously reported that the fiber-shaped TISMO, morphologically similar to asbestos, can induce a severe mesothelial reaction in A/J mice, it is important to clarify any strain differences. In the present study, female A/J, C3H/HeN, ICR and C57BL/6 mice were therefore employed as test strains. At the beginning of the experiment, all mice underwent a left thoracotomy and direct administration of 3mg of TISMO particles suspended in 0.2ml saline into the left thorax. The experiment was terminated after 21 weeks and all groups were sacrificed and the mesothelium and main organs were examined histopathologically. To contribute to mechanistic analysis, iron staining with Berlin blue and Turnbull's blue, and immunostaining for calretinin were also performed. The present experiment demonstrated only minor strain differences in the degree of pleural reaction to TISMO. However, there was clear variation in the iron and lymphocyte accumulation in the pleura and in the liver. This difference in response to TISMO fibers in vivo is important information when considering the development of mesothelioma as an animal model and the extrapolation to human risk from such animal studies.
ISSN:0940-2993
1618-1433
DOI:10.1016/j.etp.2013.01.006