Molecular characterization of bacterial and archaeal communities in a full-scale anaerobic reactor treating corn straw

•The anaerobic degradation pattern of corn straw for biogas production was investigated.•Besides volatile acids, aromatic compounds are intermediates in anaerobic degradation.•Hydrolytic and fermentative microorganisms dominate the bacterial community.•High proportion of syntrophic propionate and ar...

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Bibliographic Details
Published in:Bioresource technology 2013-09, Vol.143, p.512-518
Main Authors: Qiao, Jiang-Tao, Qiu, Yan-Ling, Yuan, Xian-Zheng, Shi, Xiao-Shuang, Xu, Xiao-Hui, Guo, Rong-Bo
Format: Article
Language:English
Subjects:
16S rRNA gene clone library
Agronomy. Soil science and plant productions
Anaerobic degradation
Anaerobiosis
Archaea - genetics
Archaea - metabolism
Aromatic compounds
Bacteria
Bacteria - genetics
Bacteria - metabolism
Base Sequence
Biological and medical sciences
Bioreactors
Biotechnology
Communities
Corn
Corn straw
Degradation
DNA Primers
Food industries
Fundamental and applied biological sciences. Psychology
General agronomy. Plant production
Methods. Procedures. Technologies
Microbial community
Microorganisms
Phylogeny
Polymerase Chain Reaction
Reactors
RNA, Ribosomal, 16S - genetics
Straw
Use and upgrading of agricultural and food by-products. Biotechnology
Use of agricultural and forest wastes. Biomass use, bioconversion
Various methods and equipments
Zea mays
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Summary:•The anaerobic degradation pattern of corn straw for biogas production was investigated.•Besides volatile acids, aromatic compounds are intermediates in anaerobic degradation.•Hydrolytic and fermentative microorganisms dominate the bacterial community.•High proportion of syntrophic propionate and aromatic acids degrading bacteria were detected.•Hydrogenotrophic methanogens were more dominant than aceticlastic methanogens. A 16S rRNA gene-based method was used to characterize the structure of bacterial and archaeal communities in a full-scale, anaerobic reactor treating corn straw. Degradability experiment indicated biogas slurry had high microbial activity, the TS removal rate was 53% and the specific methanogenic activity was 86mLCH4gVSS−1d−1. During anaerobic degradation of corn straw, volatile acids and aromatic compounds (p-cresol, phenylpropionate, phenol and benzoate) were detected as transient intermediates. Phylogenetic analysis revealed bacterial community exhibited high diversity, 69 bacterial phylotypes in 13 phyla were identified. Firmicutes (48.3%), Chloroflexi (20.1%), Actinobacteria (9.1%), Bacteroidetes (7.7%), and Proteobacteria (7.2%) represented the most abundant bacterial phyla. Hydrolytic and fermentative bacteria were major bacterial populations. Moreover, a relatively high proportion of syntrophic propionate and aromatic compounds degrading bacteria were detected. In the archaeal clone library, 11 archaeal phylotypes affiliated with two phyla of Crenarchaeota (10%) and Euryarchaeota (90%) were identified.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2013.06.014