BOD biosensor based on the yeast Debaryomyces hansenii immobilized in poly(vinyl alcohol) modified by N-vinylpyrrolidone

•The yeast Debaryomyces hansenii was used as the basis of a BOD biosensor.•The yeast is immobilized in poly(vinyl alcohol) modified by N-vinylpyrrolidone.•The modification provides for a high sensitivity and stability of the bioreceptor. An amperometric biosensor for assessing the biochemical oxygen...

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Bibliographic Details
Published in:Enzyme and microbial technology 2013-09, Vol.53 (4), p.257-262
Main Authors: Arlyapov, V.A., Yudina, N.Yu, Asulyan, L.D., Alferov, S.V., Alferov, V.A., Reshetilov, A.N.
Format: Article
Language:English
Subjects:
amino acids
biochemical oxygen demand
Biological Oxygen Demand Analysis - methods
Biosensing Techniques - methods
biosensors
BOD biosensor
carbohydrates
carboxylic acids
Cells, Immobilized - metabolism
Debaromyces - metabolism
Debaryomyces hansenii
Immobilization in poly(vinyl alcohol) modified by N-vinylpyrrolidone
nitrophenols
Polyvinyl Alcohol
Pyrrolidinones
salts
surfactants
temperature
toxicity
Waste Water - analysis
wastewater
Yeast Debaryomyces hansenii
yeasts
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Summary:•The yeast Debaryomyces hansenii was used as the basis of a BOD biosensor.•The yeast is immobilized in poly(vinyl alcohol) modified by N-vinylpyrrolidone.•The modification provides for a high sensitivity and stability of the bioreceptor. An amperometric biosensor for assessing the biochemical oxygen demand (BOD) was formed by immobilizing Debaryomyces hansenii VKM Y-2482 yeast cells in poly(vinyl alcohol) modified by N-vinylpyrrolidone. Modification provided for a high sensitivity and stability of the bioreceptor. A high oxidative activity of the receptor element and the absence of any toxic effect of assayed compounds were shown for 34 substrates (alcohols, carbohydrates, carboxylic acids, amino acids, nitrophenols and surfactants) that may occur in wastewaters. Estimates of the measurement range and region of the linear dependence of signals on the BOD level, pH and temperature sensitivities, dependences of signals on concentrations of salts, stability, Michaelis kinetic constants and assay rates were obtained. The BOD values determined by the biosensor in assayed wastewater samples were shown to have a high correlation with those obtained by the standard dilution method.
ISSN:0141-0229
1879-0909
DOI:10.1016/j.enzmictec.2013.05.004