Simultaneous Quantification of Flavonoids and Phenolic Acids in Plant Materials by a Newly Developed Isocratic High-Performance Liquid Chromatography Approach

A simple reversed-phase high-performance liquid chromatographic (RP-HPLC) method based on isocratic elution has been developed and validated for the simultaneous quantitation of flavonols (myricetin, quercetin, kaempferol, and isorhamnetin), flavones (luteolin and apigenin), and phenolic acids (chlo...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2010-10, Vol.58 (20), p.10812-10816
Main Authors: Haghi, Ghasem, Hatami, Alireza
Format: Article
Language:English
Subjects:
acid hydrolysis
Analytical Methods
Biological and medical sciences
chemical constituents of plants
Chromatography, High Pressure Liquid - methods
flavones
flavonols
Flavonols - analysis
Food industries
Fruit - chemistry
fruits (food)
Fundamental and applied biological sciences. Psychology
high performance liquid chromatography
Hydroxybenzoates - analysis
isocratic elution
isocratic high performance liquid chromatography
laboratory techniques
medicinal plants
phenolic acids
phytochemicals
Plant Extracts - analysis
Plants, Medicinal - chemistry
quantitative analysis
vegetables
Vegetables - chemistry
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Summary:A simple reversed-phase high-performance liquid chromatographic (RP-HPLC) method based on isocratic elution has been developed and validated for the simultaneous quantitation of flavonols (myricetin, quercetin, kaempferol, and isorhamnetin), flavones (luteolin and apigenin), and phenolic acids (chlorogenic, caffeic, ellagic, and rosmarinic acids) as important constituents in fruits, vegetables, and medicinal plants. Analysis was achieved on a C18 column at ambient temperature. The wavelengths used for the detection of flavonols, flavones, and phenolic acids were 370, 350, and 325 nm, respectively. After acid hydrolysis, the flavonoid aglycones were quantified straightforwardly in 20 dry herbal samples. The plants with the highest flavonoids were Rosa damascena, Solidago virgaurea, Ginkgo biloba, and Camellia sinensis. The contents of flavonoids aglycons ranged from 0.54 to 11.10 mg/g, from 0.03 to 14.80 mg/g, from 0.19 to 2.76 mg/g, from 0.15 to 2.36 mg/g, from 0.27 to 2.05 mg/g, and from 0.42 to 1.82 mg/g for quercetin, kaempferol, isorhamnetin, luteolin, apigenin, and myricetin in dry plant samples, respectively.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf102175x