Adhesion Domain of Human T11 (CD2) is Encoded by a Single Exon

The 50-kDa T11 (CD2) T-lymphocyte surface glycoprotein facilitates physical adhesion between T-lineage cells and their cognate cellular counterparts (cytotoxic T-lymphocytes-target cells, helper T lymphocytes-antigen-presenting cells, or thymocytes-thymic epithelium) as well as signaling through the...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1988-07, Vol.85 (14), p.5176-5180
Main Authors: Richardson, Neil E., Chang, Hsiu-Ching, Brown, Nicholas R., Hussey, Rebecca E., Sayre, Peter H., Reinherz, Ellis L.
Format: Article
Language:English
Subjects:
550201 - Biochemistry- Tracer Techniques
AMINO ACID SEQUENCE
AMINO ACIDS
ANIMAL CELLS
Antibodies
Antigens, Differentiation - genetics
Antigens, Differentiation - immunology
BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CARBOXYLIC ACIDS
CD2 Antigens
Cell Adhesion
CHROMATOGRAPHY
Chromatography, Affinity
Chromatography, High Pressure Liquid
Complementary DNA
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DNA, Recombinant
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
Electrophoresis, Polyacrylamide Gel
Epitopes
EVEN-ODD NUCLEI
Exons
GLUCOPROTEINS
GLYCOPROTEINS
Glycosylation
Humans
IMMUNOLOGY
Immunosorbent Techniques
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LEUKOCYTES
LIGHT NUCLEI
LIPOTROPIC FACTORS
LIQUID COLUMN CHROMATOGRAPHY
LYMPHOCYTES
Macromolecular Substances
MATERIALS
METHIONINE
Molecular Sequence Data
MOLECULAR STRUCTURE
Molecular Weight
Molecules
Monoclonal antibodies
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
Peptide Fragments
PROTEINS
RADIOISOTOPES
Receptors, Immunologic - genetics
Receptors, Immunologic - immunology
Rosette Formation
SACCHARIDES
SEPARATION PROCESSES
SOMATIC CELLS
Structure-Activity Relationship
SULFUR 35
SULFUR ISOTOPES
T lymphocytes
T-Lymphocytes - immunology
Trypsin
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Description
Summary:The 50-kDa T11 (CD2) T-lymphocyte surface glycoprotein facilitates physical adhesion between T-lineage cells and their cognate cellular counterparts (cytotoxic T-lymphocytes-target cells, helper T lymphocytes-antigen-presenting cells, or thymocytes-thymic epithelium) as well as signaling through the antigen-specific T3-Ti receptor complex. To examine the relationship between the structure and function of the T11 molecule, we have utilized a baculoviral expression system to produce milligram quantities of the hydrophilic extracellular T11 segment. Enzyme cleavage, microsequencing, and HPLC analyses of the expressed protein in conjunction with genomic cloning information show that the domain involved in cellular adhesion is encoded by a single 321-base-pair exon.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.85.14.5176