Effect of sex hormones on levels of mRNAs coding for proteins involved in lipid metabolism in macrophages

The effects of sex hormones estradiol (E2), testosterone (Te), and 5α-dihydrotestosterone (DT) on cholesterol accumulation induced by modified low density lipoproteins (LDL) in macrophages differentiated from human peripheral blood monocytes and on the levels of mRNAs coding for proteins involved in...

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Bibliographic Details
Published in:Biochemistry (Moscow) 2013-12, Vol.78 (12), p.1342-1353
Main Authors: Shchelkunova, T. A., Morozov, I. A., Rubtsov, P. M., Samokhodskaya, L. M., Andrianova, I. V., Rudimov, E. G., Sobenin, I. A., Orekhov, A. N., Smirnov, A. N.
Format: Article
Language:English
Subjects:
Adult
Biochemistry
Biomedical and Life Sciences
Biomedicine
Bioorganic Chemistry
Cholesterol
Cholesterol - metabolism
Dihydrotestosterone - pharmacology
Estradiol - pharmacology
Female
Gene expression
Gene Expression - drug effects
Genetic aspects
Gonadal Steroid Hormones - pharmacology
Hormones
Hormones, Sex
Humans
Life Sciences
Lipid metabolism
Lipid Metabolism - genetics
Lipoproteins, LDL - pharmacology
Macrophages
Macrophages - drug effects
Macrophages - metabolism
Male
Messenger RNA
Metabolism
Microbiology
Middle Aged
Physiological aspects
Polymerase chain reaction
Proteins
Proteins - genetics
Proteins - metabolism
Receptors, Androgen - genetics
Receptors, Androgen - metabolism
Ribonucleic acid
RNA
RNA, Messenger - metabolism
Testosterone - pharmacology
Young Adult
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Summary:The effects of sex hormones estradiol (E2), testosterone (Te), and 5α-dihydrotestosterone (DT) on cholesterol accumulation induced by modified low density lipoproteins (LDL) in macrophages differentiated from human peripheral blood monocytes and on the levels of mRNAs coding for proteins involved in lipid metabolism have been studied. All three hormones at physiological concentrations (1 nM) are capable of reducing cholesterol accumulation in cells. The treatment of cells with modified and native (not inducing cholesterol accumulation) LDL results in similar alterations in the expression of several mRNAs aimed primarily at homeostatic regulation of lipid metabolism. These alterations depend on the sex of macrophage donors and in some cases are even reversed in cells obtained from male and female donors. The cells not treated with modified LDL have no significant gender differences in the expression of the examined mRNAs. Hormones, either independently or in combination with the modified LDL, influence the levels of some mRNAs, and each hormone shows an individual range of effects. Correlation analysis of changes in mRNA content in the cells showed that the hormones may interfere with coordination of gene expression. Hormone action leads to: (1) reduced coupling of the content of individual mRNAs with their initial levels in the control cells; (2) reduced coupling of different mRNA levels; (3) regrouping of mRNAs between the clusters; and (4) changes in the number of factors that determine the correlation links between mRNAs. The data show that sex hormones may have impact on the level of expression of certain genes and, in particular, on the coordination of gene expression in macrophages.
ISSN:0006-2979
1608-3040
DOI:10.1134/S0006297913120043