Differential expression of VGLUT1 or VGLUT2 in the trigeminothalamic or trigeminocerebellar projection neurons in the rat

The vesicular glutamate transporters, VGLUT1 and VGLUT2, reportedly display complementary distribution in the rat brain. However, co-expression of them in single neurons has been reported in some brain areas. We previously found co-expression of VGLUT1 and VGLUT2 mRNAs in a number of single neurons...

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Published in:Brain Structure and Function 2014-01, Vol.219 (1), p.211-229
Main Authors: Ge, Shun-Nan, Li, Zhi-Hong, Tang, Jun, Ma, Yunfei, Hioki, Hiroyuki, Zhang, Ting, Lu, Ya-Cheng, Zhang, Fu-Xing, Mizuno, Noboru, Kaneko, Takeshi, Liu, Ying-Ying, Lung, Mandy Siu Yu, Gao, Guo-Dong, Li, Jin-Lian
Format: Article
Language:English
Subjects:
Animals
Axons
Biomedical and Life Sciences
Biomedicine
Biotin - analogs & derivatives
Biotin - metabolism
Brain
Cell Biology
Cerebellum - cytology
Dextrans - metabolism
Fluorescence in situ hybridization
Glutamic Acid - metabolism
Male
Microinjections
MicroRNAs
Microscopy, Confocal
Microscopy, Electron, Scanning Transmission
Neural Pathways - physiology
Neurology
Neurons
Neurons - metabolism
Neurons - ultrastructure
Neurosciences
Original Article
Rats
Rats, Sprague-Dawley
RNA, Messenger - metabolism
Rodents
Stilbamidines - metabolism
Thalamus - cytology
Trigeminal Nuclei - cytology
Vesicular Glutamate Transport Protein 1 - genetics
Vesicular Glutamate Transport Protein 1 - metabolism
Vesicular Glutamate Transport Protein 1 - ultrastructure
Vesicular Glutamate Transport Protein 2 - genetics
Vesicular Glutamate Transport Protein 2 - metabolism
Vesicular Glutamate Transport Protein 2 - ultrastructure
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Summary:The vesicular glutamate transporters, VGLUT1 and VGLUT2, reportedly display complementary distribution in the rat brain. However, co-expression of them in single neurons has been reported in some brain areas. We previously found co-expression of VGLUT1 and VGLUT2 mRNAs in a number of single neurons in the principal sensory trigeminal nucleus (Vp) of the adult rat; the majority of these neurons sent their axons to the thalamic regions around the posteromedial ventral nucleus (VPM) and the posterior nuclei (Po). It is well known that trigeminothalamic (T-T) projection fibers arise not only from the Vp but also from the spinal trigeminal nucleus (Vsp), and that trigeminocerebellar (T-C) projection fibers take their origins from both of the Vp and Vsp. Thus, in the present study, we examined the expression of VGLUT1 and VGLUT2 in Vp and Vsp neurons that sent their axons to the VPM/Po regions or the cortical regions of the cerebellum. For this purpose, we combined fluorescence in situ hybridization (FISH) histochemistry with retrograde tract-tracing; immunofluorescence histochemistry was also combined with anterograde tract-tracing. The results indicate that glutamatergic Vsp neurons sending their axons to the cerebellar cortical regions mainly express VGLUT1, whereas glutamatergic Vsp neurons sending their axons to the thalamic regions express VGLUT2. The present data, in combination with those of our previous study, indicate that glutamatergic Vp neurons projecting to the cerebellar cortical regions express mainly VGLUT1, whereas the majority of glutamatergic Vp neurons projecting to the thalamus co-express VGLUT1 and VGLUT2.
ISSN:1863-2653
1863-2661
0340-2061
DOI:10.1007/s00429-012-0495-1