Bleomycin inhibits adipogenesis and accelerates fibrosis in the subcutaneous adipose layer through TGF-β1

Systemic sclerosis [scleroderma (SSc)]‐associated skin fibrosis is characterized by increased fibrosis in the dermis and a reduction in the thickness of the subcutaneous adipose tissue layer. Although many studies have examined fibrosis in SSc, only a few studies have focused on the associated reduc...

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Bibliographic Details
Published in:Experimental dermatology 2013-11, Vol.22 (11), p.769-771
Main Authors: Ohgo, Shiro, Hasegawa, Seiji, Hasebe, Yuichi, Mizutani, Hiroshi, Nakata, Satoru, Akamatsu, Hirohiko
Format: Article
Language:English
Subjects:
Adipocytes - cytology
adipogenesis
Adipogenesis - drug effects
Adipose Tissue - metabolism
Animals
Antibiotics, Antineoplastic - chemistry
bleomycin
Bleomycin - chemistry
Cell Differentiation
Collagen - biosynthesis
Culture Media, Conditioned - chemistry
Disease Models, Animal
Fibroblasts - cytology
Fibroblasts - metabolism
Fibrosis - metabolism
Fibrosis - pathology
Humans
Mice
Scleroderma, Systemic - pathology
Skin - drug effects
Skin - metabolism
Skin - pathology
skin fibrosis
systemic sclerosis
TGF-β1
Transforming Growth Factor beta1 - metabolism
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Summary:Systemic sclerosis [scleroderma (SSc)]‐associated skin fibrosis is characterized by increased fibrosis in the dermis and a reduction in the thickness of the subcutaneous adipose tissue layer. Although many studies have examined fibrosis in SSc, only a few studies have focused on the associated reduction in the thickness of the subcutaneous adipose tissue layer. In this study, we investigated the effects of SSc‐induced fibrosis on adipose tissue. We found that bleomycin suppresses adipogenesis in adipose‐derived stem cells (ASCs) and stimulates ASCs to express transforming growth factor β1 (TGF‐β1), which suppresses adipogenesis and promotes fibrosis. Furthermore, we found that adipocyte‐conditioned medium suppressed collagen synthesis by fibroblasts in fibrosis‐like conditions. We concluded that in the skin affected by bleomycin‐induced fibrosis, increased TGF‐β1 expression suppresses adipogenesis and promotes adipocyte fibrosis. It was also suggested that adipocytes have an inhibitory effect on the progression of fibrosis.
ISSN:0906-6705
1600-0625
DOI:10.1111/exd.12256