Photosynthetic apparatus in chilling sensitive plants. I. Reactivation of Hill reaction activity inhibited on the cold and dark storage of detached leaves and intact plants

Chloroplast isolated from the detached leaves of chilling-sensitive plants — Phaseolus vulgaris L., Cucumis sativus L., and Lycopersicon esculentum Mill. — stored in the cold for 2—4 days in the dark exhibit an almost complete loss of Hill reaction activity, which on illumination of leaves is restor...

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Bibliographic Details
Published in:Planta 1978, Vol.140 (2), p.121-128
Main Authors: Kaniuga, Z, Sochanowicz, B, Zabek, J, Krzystyniak, K
Format: Article
Language:English
Subjects:
beans
Chloroplasts
Cold storage
Cooling
Cucumbers
Fatty acids
Leaves
Low temperature
Nonesterified fatty acids
Plants
Spinach
tomatoes
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Summary:Chloroplast isolated from the detached leaves of chilling-sensitive plants — Phaseolus vulgaris L., Cucumis sativus L., and Lycopersicon esculentum Mill. — stored in the cold for 2—4 days in the dark exhibit an almost complete loss of Hill reaction activity, which on illumination of leaves is restored to almost the original level. In contrast, illumination of either chloroplast suspensions or homogenates from leaves stored in the cold in the dark does not cause restoration of electron transport. Cold and dark storage of leaves of chilling-sensitive plants affects the electron transport before the site of electron donation by diphenylcarbazide and results in an increased sensitivity of the Hill reaction of isolated chloroplasts to exogenous linolenic acid. Illumination of leaves reverses these processes. When tomato plants are exposed to 0° C in intermittent light, Hill reaction activity is not affected while dark storage either at 0° C or 25° C results in a significant decrease of Hill reaction activity after 2—3 days followed by the restoration of electron transport to the original level after 1 or 2 days of the prolonged dark storage of plants. When tomato plants are stored either at 0° C in intermittent light, at 0° C in dark, or at 25° C in dark the sensitivity of the Hill reaction to exogenous linolenic acid remains increased despite a significant restoration of this activity. In conclusion, both darkness and the detachment of leaves from the plant are more effective than cold treatment in damaging photosystem II whereas both light and intact structure of the cell are required for restoration of Hill reaction activity in chloroplasts following cold and dark storage of detached leaves.
ISSN:0032-0935
1432-2048
DOI:10.1007/BF00384910