The quenching characteristics of potassium iridic chloride and their meaning for the origin of chlorophyll fluorescence components

To understand the origins of the different lifetime components of photosystem 2 (PS2) chlorophyll (Chl) fluorescence we have studied their susceptibility to potassium iridic chloride (K2IrCl6) which has been shown to bleach antenna pigments of photosynthetic bacteria (Loach et al. 1963). The additio...

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Bibliographic Details
Published in:Photosynthesis research 1989-05, Vol.20 (2), p.173-189
Main Authors: BOUSSAC, A, HODGES, M, BRIANTAIS, J.-M, MOYA, I
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell biochemistry
Cell physiology
Fundamental and applied biological sciences. Psychology
Plant physiology and development
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Summary:To understand the origins of the different lifetime components of photosystem 2 (PS2) chlorophyll (Chl) fluorescence we have studied their susceptibility to potassium iridic chloride (K2IrCl6) which has been shown to bleach antenna pigments of photosynthetic bacteria (Loach et al. 1963). The addition of K2IrCl6 to PS2 particles gives rise to a preferential quenching of the variable Chl fluorescence (Fv). At concentrations lower than 20 μM, this is brought about mainly by a decrease in the yield, but not in the lifetime, of the slowest component when all the PS2 reaction centres are closed (FM). The yield of the middle and fast decays are not significantly altered. This type of quenching is not seen with DNB. The iridate-induced quenching of the initial fluorescence level (F0) is due to a proportional decrease in the yield and lifetime of the three components and correlates with the observed modification in the relative quantum yield of oxygen evolution. In this concentration range a bleaching of Chl a is seen. At higher iridate levels, greater than 20 μM, a proportional decrease in the lifetimes and yields of the three kinetic components is seen at FM. These changes are associated with a carotenoid bleaching. In isolated light harvesting Chl a/b complexes of PS2 (LHC2), iridate addition converts a 4 ns decay into a 200 ps emission and both types of bleaching are observed. By also measuring the rate of PS2 trap closure versus iridate concentration, we have discussed the results in terms of excitation energy transfer.
ISSN:0166-8595
1573-5079
DOI:10.1007/BF00034125