Different molecular weight hyaluronic acid effects on human macrophage interleukin 1β production

ABSTRACT This study examined the effect of hyaluronan (HA) molecular weight on immune response. HA with molecular weights ranging from the unitary disaccharide unit (400 Da) up to 1.7 × 106 Da and with very low endotoxin contamination level (less than 0.03 EU/mg) was used. Primary human monocyte/mac...

Full description

Saved in:
Bibliographic Details
Published in:Journal of biomedical materials research. Part A 2014-02, Vol.102 (2), p.305-314
Main Authors: Baeva, Larissa F., Lyle, Daniel B., Rios, Maria, Langone, John J., Lightfoote, Marilyn M.
Format: Article
Language:English
Subjects:
Adjuvants, Immunologic - pharmacology
Animals
Biological and medical sciences
biomaterials
Cell Line
Humans
hyaluronic acid
Hyaluronic Acid - pharmacology
inflammation
Inflammation - chemically induced
Inflammation - metabolism
interleukin 1β
Interleukin-1beta - biosynthesis
macrophage
Macrophages - cytology
Macrophages - metabolism
Materials Testing
Medical sciences
Molecular Weight
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Technology. Biomaterials. Equipments
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:ABSTRACT This study examined the effect of hyaluronan (HA) molecular weight on immune response. HA with molecular weights ranging from the unitary disaccharide unit (400 Da) up to 1.7 × 106 Da and with very low endotoxin contamination level (less than 0.03 EU/mg) was used. Primary human monocyte/macrophage cultures were assayed for IL‐1β production under a variety of inflammatory conditions with or without HA. Under the highest inflammatory states, production of interleukin 1β (IL‐1β) was suppressed in the presence of high molecular weight hyaluronan (HMW‐HA) and in the presence of low molecular weight hyaluronan (LMW‐HA) at mg/mL concentrations. There was variability in the sensitivity of the response to HA fragments with MW below 5000 Da at micromolar concentrations. There was variability in IL‐1β cytokine productions from donor to donor in unstimulated human cell cultures. This study supplements our previous published study that investigated the immunogenic effect of HA molecular weights using murine cell line RAW264.6, rat splenocytes, and rat adherent differentiated primary macrophages. These data support the hypothesis that if the amount of endotoxin is reduced to an extremely low level, LMW‐HA may not directly provoke normal tissue macrophage‐mediated inflammatory reactions. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 305–314, 2014.
ISSN:1549-3296
1552-4965
DOI:10.1002/jbm.a.34704