Vigna angularis inhibits IL-6-induced cellular signalling and ameliorates collagen-induced arthritis

The present study was conducted in order to assess whether extracts or isolated compounds from Vigna angularis were able to suppress IL-6 signalling and to show the therapeutic effect on collagen-induced arthritis (CIA) in mice. The effect of V. angularis on IL-6 signalling was studied by measuring...

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Published in:Rheumatology (Oxford, England) England), 2014-01, Vol.53 (1), p.56-64
Main Authors: Oh, Hyun-Mee, Lee, Seung Woong, Yun, Bo Ra, Hwang, Byung Soon, Kim, So Nam, Park, Chan Sun, Jeoung, See-Hwa, Kim, Hyun-Kyu, Lee, Woo Song, Rho, Mun-Chual
Format: Article
Language:English
Subjects:
Administration, Oral
Animals
Arthritis, Experimental - drug therapy
Arthritis, Experimental - genetics
Arthritis, Experimental - pathology
Blotting, Western
Cattle
Collagen - toxicity
Gene Expression Regulation
Humans
Interleukin-6 - pharmacology
Male
Mice
Mice, Inbred DBA
Plant Extracts - administration & dosage
Plant Extracts - therapeutic use
RNA - genetics
Signal Transduction - drug effects
STAT3 Transcription Factor - biosynthesis
STAT3 Transcription Factor - genetics
Tumor Cells, Cultured
Tumor Necrosis Factor-alpha - pharmacology
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Summary:The present study was conducted in order to assess whether extracts or isolated compounds from Vigna angularis were able to suppress IL-6 signalling and to show the therapeutic effect on collagen-induced arthritis (CIA) in mice. The effect of V. angularis on IL-6 signalling was studied by measuring Stat3-dependent luciferase activity, expression of inflammation-related genes, and phosphorylation of Janus kinase 2 (JAK2), signal transducer and activator of transcription 3 (STAT3) and extracellular signal-regulated kinase (ERK) induced by IL-6. CIA was induced by immunizing with bovine type II collagen. V. angularis extract (VAE) was administrated orally at 50 and 100 mg/kg from day 1 to day 28. Induction of arthritis was evaluated with a visual scoring system and histological analysis. Extracts or two triterpenoid compounds from V. angularis showed potent inhibitory effects on pSTAT3-inducible luciferase activity, STAT3 tyrosine phosphorylation and the expression of inflammation-related genes induced by IL-6. Administration of VAE significantly suppressed the progression of CIA, accompanied by a reduced antibody response to type II collagen and protection from tissue damage in knee joints. Administration of VAE has a therapeutic effect on CIA and this effect is associated with the inhibitory activity on IL-6/STAT3 signalling. These results suggest that extracts or compounds from V. angularis could be a useful treatment for diseases related to IL-6, including RA.
ISSN:1462-0324
1462-0332
DOI:10.1093/rheumatology/ket302