Anti- beta -interferon antibodies inhibit the increased expression of HLA-B7 mRNA in tumor necrosis factor-treated human fibroblasts: Structural studies of the beta sub(2) interferon involved

Recombinant Escherichia coli -derived human tumor necrosis factor (TNF) induces the 1.3-kilobase beta sub(2) interferon (IFN- beta sub(2)) mRNA in human diploid fibroblasts (FS-4 strain). IFN- beta sub(2) is serologically related to the well-characterized IFN- beta sub(1) (respective antisera cross-...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1986-01, Vol.83 (23), p.8957-8961
Main Authors: May, L T, Helfgott, D C, Sehgal, P B
Format: Article
Language:English
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Recombinant Escherichia coli -derived human tumor necrosis factor (TNF) induces the 1.3-kilobase beta sub(2) interferon (IFN- beta sub(2)) mRNA in human diploid fibroblasts (FS-4 strain). IFN- beta sub(2) is serologically related to the well-characterized IFN- beta sub(1) (respective antisera cross-neutralize the heterologous protein). Polyclonal and monoclonal anti-IFN- beta antibodies inhibit the increase in class I HLA gene expression (HLA-B7 mRNA) in TNF-treated FS-4 cells suggesting that TNF-induced IFN- beta sub(2) mediates the enhancing effect of TNF on HLA gene expression in human fibroblasts. The hydropathic index plots across these segments in the two proteins are also strikingly similar. The region of similarity between IFN- beta sub(1) and - beta sub(2) includes a section that is also highly conserved in all IFN- alpha species sequenced. Thus IFN- beta sub(2) shares structural similarities with other human interferons that also preferentially increase class I HLA gene expression.
ISSN:0027-8424