Raman spectrometric discrimination of flexirubin pigments from two genera of Bacteroidetes

Abstract Flexirubins are specific polyene pigments produced by several genera of Bacteroidetes. Colonies and cell extracts of Flavobacterium johnsoniae and Flexibacter elegans have been investigated by Raman spectroscopy to show that this fast and non-destructive technique can be used to differentia...

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Bibliographic Details
Published in:FEMS microbiology letters 2013-11, Vol.348 (2), p.97-102
Main Authors: Jehlička, Jan, Osterrothová, Kateřina, Oren, Aharon, Edwards, Howell G. M.
Format: Article
Language:English
Subjects:
Bacteroidetes - chemistry
Biological and medical sciences
chemotaxonomy
dialkylated resorcinol
Flavobacterium johnsoniae
Flexibacter
Fundamental and applied biological sciences. Psychology
identification
Microbiology
Pigments, Biological - analysis
Pigments, Biological - chemistry
Polyenes - analysis
Polyenes - chemistry
p‐hydroxyphenyl group
Spectrum Analysis, Raman
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Summary:Abstract Flexirubins are specific polyene pigments produced by several genera of Bacteroidetes. Colonies and cell extracts of Flavobacterium johnsoniae and Flexibacter elegans have been investigated by Raman spectroscopy to show that this fast and non-destructive technique can be used to differentiate these pigments from carotenoids and to compare the flexirubin content of the two microorganisms. The presence or absence of certain distinguishing features in the CH combination band region at 2500–2750 cm−1 can assist in the discrimination between the two flexirubins investigated. Raman spectroscopy is thus a suitable tool not only to detect flexirubin pigments in bacterial cells, but also to further characterize the pigments present in members of the Bacteroidetes genera that are rich in flexirubins.
ISSN:0378-1097
1574-6968
DOI:10.1111/1574-6968.12243