Nano-Chemical Infrared Imaging of Membrane Proteins in Lipid Bilayers

The spectroscopic characterization of biomolecular structures requires nanometer spatial resolution and chemical specificity. We perform full spatio-spectral imaging of dried purple membrane patches purified from Halobacterium salinarum with infrared vibrational scattering-type scanning near-field o...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2013-12, Vol.135 (49), p.18292-18295
Main Authors: Berweger, Samuel, Nguyen, Duc M, Muller, Eric A, Bechtel, Hans A, Perkins, Thomas T, Raschke, Markus B
Format: Article
Language:English
Subjects:
Lipid Bilayers
Membrane Proteins - chemistry
Microscopy, Atomic Force
Nanotechnology
Spectrophotometry, Infrared - methods
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Summary:The spectroscopic characterization of biomolecular structures requires nanometer spatial resolution and chemical specificity. We perform full spatio-spectral imaging of dried purple membrane patches purified from Halobacterium salinarum with infrared vibrational scattering-type scanning near-field optical microscopy (s-SNOM). Using near-field spectral phase contrast based on the Amide I resonance of the protein backbone, we identify the protein distribution with 20 nm spatial resolution and few-protein sensitivity. This demonstrates the general applicability of s-SNOM vibrational nanospectroscopy, with potential extension to a wide range of biomolecular systems.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja409815g