Thyroglobulin gene expression is regulated by insulin and insulin-like growth factor I, as well as thyrotropin, in FRTL-5 thyroid cells

In FRTL-5 thyroid cells depleted of thyrotropin, insulin, and serum for 3 days, growth and synthesis of thyroglobulin are inhibited. Readdition of insulin or IGF-I in the absence of thyrotropin stimulates thyroglobulin synthesis or thyroglobulin mRNA level by 2-4-fold but has no effect on cell growt...

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Bibliographic Details
Published in:The Journal of biological chemistry 1987-03, Vol.262 (9), p.4048-4052
Main Authors: Santisteban, P., Kohn, L.D., Di Lauro, R.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell Division - drug effects
Cell Line
Drug Interactions
Fundamental and applied biological sciences. Psychology
Gene expression
Gene Expression Regulation - drug effects
Insulin - pharmacology
Insulin-Like Growth Factor I - pharmacology
Kinetics
Molecular and cellular biology
Molecular genetics
RNA, Messenger - biosynthesis
Somatomedins - pharmacology
Thyroglobulin - genetics
Thyrotropin - pharmacology
Transcription, Genetic - drug effects
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Summary:In FRTL-5 thyroid cells depleted of thyrotropin, insulin, and serum for 3 days, growth and synthesis of thyroglobulin are inhibited. Readdition of insulin or IGF-I in the absence of thyrotropin stimulates thyroglobulin synthesis or thyroglobulin mRNA level by 2-4-fold but has no effect on cell growth. The half-maximal effects of insulin and IGF-I are at 100 and 10 ng/ml, respectively. In both cases, the increased mRNA levels are accompanied by an increase in transcription rate. Maximally effective concentrations of insulin or IGF-I are additive with thyrotropin in increasing thyroglobulin mRNA levels and in increasing transcription; in contrast, the effect of insulin is not additive with IGF-I.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)61309-2