Comparative analysis of peroxiredoxin activation in the brown macroalgae Scytosiphon gracilis and Lessonia nigrescens (Phaeophyceae) under copper stress

Among thiol‐dependent peroxidases (TDPs) peroxiredoxins (PRXs) standout, since they are enzymes capable of reducing hydrogen peroxide, alkylhydroperoxides and peroxynitrite, and have been detected in a proteomic study of the copper‐tolerant species Scytosiphon gracilis. In order to determine the imp...

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Bibliographic Details
Published in:Physiologia plantarum 2013-11, Vol.149 (3), p.378-388
Main Authors: Lovazzano, Carlos, Serrano, Carolina, Correa, Juan A., Contreras-Porcia, Loretto
Format: Article
Language:English
Subjects:
Biological and medical sciences
Copper - metabolism
Dithiothreitol - metabolism
Fundamental and applied biological sciences. Psychology
Glutaredoxins - metabolism
Lessonia nigrescens
Lipid Peroxides - metabolism
Peroxidases - metabolism
Peroxiredoxins - metabolism
Phaeophyceae
Phaeophyceae - enzymology
Plant physiology and development
Scytosiphon gracilis
Stress, Physiological
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Summary:Among thiol‐dependent peroxidases (TDPs) peroxiredoxins (PRXs) standout, since they are enzymes capable of reducing hydrogen peroxide, alkylhydroperoxides and peroxynitrite, and have been detected in a proteomic study of the copper‐tolerant species Scytosiphon gracilis. In order to determine the importance of these enzymes in copper‐stress tolerance, TDP activity and type II peroxiredoxin (II PRX) protein expression were compared between the opportunistic S. gracilis and the brown kelp Lessonia nigrescens, a species absent from copper‐impacted sites due to insufficient copper‐tolerance mechanisms. Individuals of both species were cultured with increasing copper concentrations (0–300 µg l−1 Cu) for 96 h and TDP activity and lipoperoxides (LPXs) were determined together with II PRX expression by immunofluorescence and Western blot analysis. The results showed that TDP activity was higher in S. gracilis than L. nigrescens in all copper concentrations, independent of the reducing agent used (dithiothreitol, thioredoxin or glutaredoxin). This activity was copper inhibited in L. nigrescens at lower copper concentrations (20 µg l−1 Cu) compared to S. gracilis (100 µg l−1 Cu). The loss of activity coincided in both species with an increase in LPX, which suggests that TDP may control LPX production. Moreover, II PRX protein levels increased under copper stress only in S. gracilis. These results suggest that in S. gracilis TDP, particularly type II peroxiredoxin (II PRX), acts as an active antioxidant barrier attenuating the LPX levels generated by copper, which is not the case in L. nigrescens. Thus, from an ecological point of view these results help explaining the inability of L. nigrescens to flourish in copper‐enriched environments.
ISSN:0031-9317
1399-3054
DOI:10.1111/ppl.12047