Auxin response factor (OsARF12), a novel regulator for phosphate homeostasis in rice (Oryza sativa)

Phosphorus (P) is crucial nutrient element for crop growth and development. However, the network pathway regulating homeostasis of phosphate (Pi) in crops has many molecular breeding unknowns. Here, we report that an auxin response factor, OsARF12, functions in Pi homeostasis. Measurement of element...

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Bibliographic Details
Published in:The New phytologist 2014-01, Vol.201 (1), p.91-103
Main Authors: Wang, SuiKang, Zhang, SaiNa, Sun, ChenDong, Xu, YanXia, Chen, Yue, Yu, ChenLiang, Qian, Qian, Jiang, De‐An, Qi, YanHua
Format: Article
Language:English
Subjects:
auxin response factor
Auxins
Breeding
Crop growth
Gene expression regulation
Gene Expression Regulation, Plant
Genes
Genes, Plant
Homeostasis
Indoleacetic Acids - metabolism
MicroRNAs
Mutants
Mutation
Nucleotide sequence
Oryza - genetics
Oryza - metabolism
Oryza sativa (rice)
OsARF12
PCR
Phenotypes
phosphate absorption and transport
phosphate homeostasis
Phosphate starvation response
Phosphates
Phosphates - metabolism
Phosphorus
Phosphorus - metabolism
Plant breeding
Plant Proteins - genetics
Plant Proteins - metabolism
Plant roots
Plant Roots - metabolism
Plants
Plants, Genetically Modified
Polymerase chain reaction
Reverse transcription
Rice
Sodium phosphate
Starvation
Transcription
Transcription Factors - genetics
Transcription Factors - metabolism
Transcriptional Activation
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Summary:Phosphorus (P) is crucial nutrient element for crop growth and development. However, the network pathway regulating homeostasis of phosphate (Pi) in crops has many molecular breeding unknowns. Here, we report that an auxin response factor, OsARF12, functions in Pi homeostasis. Measurement of element content, quantitative reverse transcription polymerase chain reaction analysis and acid phosphatases (APases) activity assay showed that the osarf12 mutant and osarf12/25 double mutant with P-intoxicated phenotypes had higher P concentrations, up-regulation of the Pi transporter encoding genes and increased APase activity under Pi-sufficient/-deficient (+Pi/−Pi, 0.32/0mM NaH2PO4) conditions. Transcript analysis revealed that Pi-responsive genes – Phosphate starvation (OsIPS)1 and OsIPS2, SYG1/Pho81/XPR1 (OsSPX1), Sulfoquinovosyldiacylglycerol 2 (OsSQD2), R2R3 MYB transcription factor (OsMYB2P-1) and Transport Inhibitor Response1 (OsTIR1) – were more abundant in the osarf12 and osarf12/25 mutants under +Pi/−Pi conditions. Knockout of OsARF12 also influenced the transcript abundances of the OsPHR2 gene and its downstream components, such as OsMiR399j, OsPHO2, OsMiR827, OsSPX-MFS1 and OsSPXMFS2. Results from −Pi/1-naphthylphthalamic acid (NPA) treatments, and auxin reporter DR5∷GUS staining suggest that root system alteration and Pi-induced auxin response were at least partially controlled by OsARF12. These findings enrich our understanding of the biological functions of OsARF12, which also acts in regulating Pi homeostasis.
ISSN:0028-646X
1469-8137
DOI:10.1111/nph.12499