A Dried Yeast Fermentate Selectively Modulates both the Luminal and Mucosal Gut Microbiota and Protects against Inflammation, As Studied in an Integrated in Vitro Approach

EpiCor, derived from Saccharomyces cerevisiae, has been shown to have immunomodulating properties in human clinical trials and in vitro. However, the underlying mechanisms behind its immune protection via the gut remain largely unknown. Therefore, the aim of this study was to use an integrated in vi...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2013-10, Vol.61 (39), p.9380-9392
Main Authors: Possemiers, Sam, Pinheiro, Iris, Verhelst, An, Van den Abbeele, Pieter, Maignien, Lois, Laukens, Debby, Reeves, Stuart G, Robinson, Larry E, Raas, Thomas, Schneider, Yves-Jacques, Van de Wiele, Tom, Marzorati, Massimo
Format: Article
Language:English
Subjects:
adhesion
anti-inflammatory activity
Anti-Inflammatory Agents - metabolism
Bacterial Adhesion
Bifidobacterium - growth & development
Bifidobacterium - immunology
Bifidobacterium - metabolism
Biological and medical sciences
Cell Line
clinical trials
Clostridium - growth & development
Clostridium - immunology
Clostridium - metabolism
Coculture Techniques
community structure
cytokines
Cytokines - antagonists & inhibitors
Cytokines - metabolism
digestive system
Enterobacteriaceae - growth & development
Enterobacteriaceae - immunology
Enterobacteriaceae - metabolism
Enterocytes - immunology
Enterocytes - metabolism
Enterocytes - microbiology
Enterocytes - secretion
Fermentation
Food industries
Fundamental and applied biological sciences. Psychology
human cell lines
Humans
Immunologic Factors - metabolism
immunomodulators
inflammation
intestinal microorganisms
Lactobacillaceae - growth & development
Lactobacillaceae - immunology
Lactobacillaceae - metabolism
Lactobacillus
metabolism
Monocytes - immunology
Monocytes - metabolism
Monocytes - microbiology
Monocytes - secretion
mucus
Mucus - secretion
pathogens
Prebiotics
Saccharomyces cerevisiae
Saccharomyces cerevisiae - metabolism
yeasts
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Summary:EpiCor, derived from Saccharomyces cerevisiae, has been shown to have immunomodulating properties in human clinical trials and in vitro. However, the underlying mechanisms behind its immune protection via the gut remain largely unknown. Therefore, the aim of this study was to use an integrated in vitro approach to evaluate the metabolism of EpiCor by the intestinal microflora, its modulating effect on the gut microbiota, and its anti-inflammatory activity on human-derived cell lines. Using the SHIME model, in combination with a mucus adhesion assay, has shown that low doses of EpiCor have a prebiotic-like modulatory effect on the luminal- and mucosa-associated microbiota. These include gradual changes in general community structure, reduction of potential pathogens, quantitative increase in lactobacilli, and qualitative modulation of bifidobacteria. Moreover, by combination of the SHIME with Caco-2 cells and Caco-2/THP1 cocultures, a significant decrease in pro-inflammatory cytokines was observed at the end of the treatment period.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf402137r