Monitoring of Phosphorus Oxide Ion for Analytical Speciation of Phosphite and Phosphate in Transgenic Plants by High-Performance Liquid Chromatography–Inductively Coupled Plasma Mass Spectrometry

Large amounts of phosphate fertilizers utilized in agriculture and their relatively poor efficiency are of high ecological and economic concern. Therefore, transgenic plants capable of metabolizing phosphite are being engineered. In support of this biotechnological task, analytical speciation of pho...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2013-07, Vol.61 (27), p.6622-6628
Main Authors: Torres Elguera, Julio Cesar, Yañez Barrientos, Eunice, Wrobel, Katarzyna, Wrobel, Kazimierz
Format: Article
Language:English
Subjects:
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
Food industries
Fundamental and applied biological sciences. Psychology
Mass Spectrometry - methods
Oxides - analysis
Oxides - metabolism
Phosphates - analysis
Phosphates - metabolism
Phosphites - analysis
Phosphites - metabolism
Phosphorus - analysis
Phosphorus - metabolism
Plants, Genetically Modified - chemistry
Plants, Genetically Modified - metabolism
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Summary:Large amounts of phosphate fertilizers utilized in agriculture and their relatively poor efficiency are of high ecological and economic concern. Therefore, transgenic plants capable of metabolizing phosphite are being engineered. In support of this biotechnological task, analytical speciation of phosphorus in biological tissues is required. In this study, plant extracts were analyzed by liquid chromatography–inductively coupled plasma mass spectrometry at m/z of elemental phosphorus and phosphorus oxide ions. Using polymeric-based anion exchange column and millimolar concentration of nitric acid in potassium phthalate mobile phase (pH 2.5), phosphite and phosphate ions were baseline resolved with retention times 6.95 ± 0.03 and 7.90 ± 0.03 min and with a total chromatographic run time 10 min. The detection limits were 1.58 and 1.74 μg P L–1 at m/z 47, as compared to 2.18 and 2.04 μg P L–1 at m/z 31, respectively. The results obtained in real world samples for the two detection modes were in good agreement, yet signal acquisition at m/z 47 enabled better precision without collision/reaction cell (RSD below 2%) as compared to RSD around 4% obtained at m/z 31 using He-pressurized cell (3.5 mL min–1).
ISSN:0021-8561
1520-5118
DOI:10.1021/jf4012278