Bovine Milk Fat Globule Membrane Proteins Are Affected By Centrifugal Washing Processes

The impact of washing on the release of proteins from the milk fat globule membrane (MFGM) was examined by applying washing procedures with different degrees of stringency to milk fat globule (MFG) surfaces in simulated milk ultrafiltrate buffer solution. Three washing methods, M1 (3000g, 5 min, thr...

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Bibliographic Details
Published in:	Journal of agricultural and food chemistry 2013-09, Vol.61 (35), p.8403-8411
Main Authors:	Zheng, Haotian, Jiménez-Flores, Rafael, Everett, David W
Format:	Article
Language:	eng
Subjects:	Animals Biological and medical sciences Cattle Centrifugation fat globules Fats - analysis fatty acid-binding proteins Food industries Fundamental and applied biological sciences. Psychology Glycolipids - chemistry Glycoproteins - chemistry Glycoproteins - ultrastructure Humans membrane proteins Membrane Proteins - chemistry Membrane Proteins - ultrastructure Microscopy, Confocal milk Milk - chemistry Milk and cheese industries. Ice creams milk fat Milk Proteins - analysis washing
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Summary:	The impact of washing on the release of proteins from the milk fat globule membrane (MFGM) was examined by applying washing procedures with different degrees of stringency to milk fat globule (MFG) surfaces in simulated milk ultrafiltrate buffer solution. Three washing methods, M1 (3000g, 5 min, three washes), M2 (3750g, 15 min, one wash), and M3 (15000g, 20 min, three washes) were chosen. MFG ζ-potential increased after M3 washing (P < 0.05), suggesting surface damage. For M1, in which the native MFG surface was least damaged, cluster of differentiation 36 (CD 36) and periodic acid schiff 6/7 proteins were more strongly bound to the MFGM compared with other major membrane proteins. For M3, CD 36 together with fatty acid-binding protein was more strongly bound to the MFGM. Washing by centrifugation and redispersal of the fat globules damaged the MFGM, with release into the aqueous phase of some membrane-associated proteins. The current results show the impact of washing processes on retention of functional MFGM proteins.
ISSN:	0021-8561 1520-5118