Polymorphic microsatellite loci for two Atlantic oyster species: Crassostrea rhizophorae and C. gasar

Using a CA/CAA enriched library screening procedure, we isolated and characterised a total of seventeen polymorphic microsatellite loci for two species of Crassostrea with recognised economic importance. Eleven microsatellite loci were developed for C. rhizophorae , a Western Atlantic species for wh...

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Bibliographic Details
Published in:Molecular biology reports 2013-12, Vol.40 (12), p.7039-7043
Main Authors: Cavaleiro, Nathalia P., Solé-Cava, Antonio M., Lazoski, Cristiano, Cunha, Haydée A.
Format: Article
Language:English
Subjects:
Animal Anatomy
Animal Biochemistry
Animals
Aquaculture
Atlantic Ocean
Biomedical and Life Sciences
Crassostrea - genetics
Gene loci
Genetic Loci - genetics
Histology
Life Sciences
Microsatellite Repeats - genetics
Molecular biology
Molecular Sequence Data
Mollusks
Morphology
Oysters
Polymorphism
Polymorphism, Genetic
Species Specificity
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Summary:Using a CA/CAA enriched library screening procedure, we isolated and characterised a total of seventeen polymorphic microsatellite loci for two species of Crassostrea with recognised economic importance. Eleven microsatellite loci were developed for C. rhizophorae , a Western Atlantic species for which no microsatellites were previously known. Another six loci were developed for C. gasar , a species that occurs on both sides of the South Atlantic, adding to the ten loci previously described for the species. The levels of polymorphism were estimated using 24 C. rhizophorae from Southeast Brazil (São Paulo) and 23 C. gasar individuals from North Brazil (Maranhão). The number of alleles per polymorphic locus varied from 3 to 27, and the observed and expected heterozygosities ranged between 0.174 and 0.958 and between 0.237 and 0.972 in C. rhizophorae and C. gasar , respectively. No linkage disequilibrium was found between any locus pair, and four of them exhibited deviations from Hardy–Weinberg expectations. Of the 17 loci developed, 8 cross-amplified in C. gigas and 13 in C. virginica . These markers are useful for evolution and population genetics studies of Crassostrea species and may provide fundamental data for the future cultivation of native oysters in Western Atlantic.
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-013-2823-9