The rate of production of uric acid by hepatocytes is a sensitive index of compromised cell ATP homeostasis

Plasma levels of uric acid, the final product of purine degradation in humans, are elevated in metabolic syndrome and are strongly associated with insulin resistance and nonalcoholic fatty liver disease (NAFLD). Hepatic and blood levels of purine metabolites (inosine, hypoxanthine, and xanthine) are...

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Published in:American journal of physiology: endocrinology and metabolism 2013-11, Vol.305 (10), p.E1255-E1265
Main Authors: Petrie, John L, Patman, Gillian L, Sinha, Ishita, Alexander, Thomas D, Reeves, Helen L, Agius, Loranne
Format: Article
Language:English
Subjects:
Adenosine triphosphatase
Adenosine Triphosphate - metabolism
Animals
Biomarkers - metabolism
Cells, Cultured
Gene expression
Health Status Indicators
Hep G2 Cells
Hepatocytes - metabolism
Homeostasis
Homeostasis - physiology
Humans
Liver diseases
Male
Metabolic Diseases - diagnosis
Metabolic Diseases - metabolism
Metabolic syndrome
Metabolites
Mice
Mice, Inbred C3H
Plasma
Rats
Rats, Wistar
Sensitivity and Specificity
Uric Acid - metabolism
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Summary:Plasma levels of uric acid, the final product of purine degradation in humans, are elevated in metabolic syndrome and are strongly associated with insulin resistance and nonalcoholic fatty liver disease (NAFLD). Hepatic and blood levels of purine metabolites (inosine, hypoxanthine, and xanthine) are also altered in pathophysiological states. We optimized a rat hepatocyte model to test the hypothesis that the production of uric acid by hepatocytes is a potential marker of compromised homeostasis of hepatocellular inorganic phosphate (Pi) and/or ATP. The basal rate of uric acid production from endogenous substrates in rat hepatocytes was comparable to that in human liver and was
ISSN:0193-1849
1522-1555
1522-1555
DOI:10.1152/ajpendo.00214.2013