High-rate spontaneous reversion to cytoplasmic male sterility in sugar beet: a characterization of the mitochondrial genomes

Among the fertile sugar beet lines with nuclear sterility maintenance genes, rf, in a homozygous recessive state, sublines capable of reverting spontaneously at a high rate to sterility were identified. Of 24 related fertile sublines studied, 6 were found to spontaneously revert to sterility with a...

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Bibliographic Details
Published in:Theoretical and applied genetics 1990-06, Vol.79 (6), p.817-824
Main Authors: Dudareva, N.A, Veprev, S.G, Popovsky, A.V, Maletsky, S.I, Gileva, I.P, Salganik, R.I
Format: Article
Language:English
Subjects:
adenosinetriphosphatase
atpa gene
Beta vulgaris
Biological and medical sciences
Classical genetics, quantitative genetics, hybrids
cox ii gene
cytochrome-c oxidase
cytoplasmic male sterility
DNA
Fundamental and applied biological sciences. Psychology
genes
Genetics of eukaryotes. Biological and molecular evolution
genome
genomics
mitochondrial DNA
mutation
Pteridophyta, spermatophyta
restriction mapping
southern blot analysis
Vegetals
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Summary:Among the fertile sugar beet lines with nuclear sterility maintenance genes, rf, in a homozygous recessive state, sublines capable of reverting spontaneously at a high rate to sterility were identified. Of 24 related fertile sublines studied, 6 were found to spontaneously revert to sterility with a frequency of about 19%. Genetic analysis confirmed the cytoplasmic nature of spontaneously arising sterility. Reversion to sterility in these sublines was accompanied by alterations in the mitochondrial genome structure: loss of the autonomously replicating minicircle c (1.3 kb) and changes in the restriction patterns of high-molecular-weight mitochondrial DNA (mtDNA). Southern hybridixation analysis with cloned minicircle c as a probe revealed no integration of this DNA molecule into the main mitochondrial and nuclear genomes of the revertants. Comparative BamHI and EcoRI restriction analysis of the mtDNA from the sterile revertants and fertile parental subline showed that the spontaneous reversion is accompanied by extensive genomic rearrangement. Southern blot analysis with cloned α-subunit of F1-ATPase (atpA) and cytochrome c oxidase subunit II (COX II) genes as probes indicated that the changes in mtDNA accompanying spontaneous reversion to sterility involved these regions. The mitochondrial genomes of the spontaneous revertants and the sterile analogue were shown to be identical.
ISSN:0040-5752
1432-2242
DOI:10.1007/BF00224251