MEIOB Targets Single-Strand DNA and Is Necessary for Meiotic Recombination: e1003784

Meiotic recombination is a mandatory process for sexual reproduction. We identified a protein specifically implicated in meiotic homologous recombination that we named: meiosis specific with OB domain (MEIOB). This protein is conserved among metazoan species and contains single-strand DNA binding si...

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Bibliographic Details
Published in:PLoS genetics 2013-09, Vol.9 (9)
Main Authors: Souquet, Benoit, Abby, Emilie, Hervé, Roxane, Finsterbusch, Friederike, Tourpin, Sophie, Bouffant, Ronan Le, Duquenne, Clotilde, Messiaen, Sébastien, Martini, Emmanuelle, Bernardino-Sgherri, Jacqueline, Toth, Attila, Habert, René, Livera, Gabriel
Format: Article
Language:English
Subjects:
Binding sites
Cell division
Chromosomes
Deoxyribonucleic acid
DNA
Flow cytometry
Genetic recombination
Genomes
Genotype & phenotype
Infertility
Metazoa
Proteins
Yeast
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Summary:Meiotic recombination is a mandatory process for sexual reproduction. We identified a protein specifically implicated in meiotic homologous recombination that we named: meiosis specific with OB domain (MEIOB). This protein is conserved among metazoan species and contains single-strand DNA binding sites similar to those of RPA1. Our studies in vitro revealed that both recombinant and endogenous MEIOB can be retained on single-strand DNA. Those in vivo demonstrated the specific expression of Meiob in early meiotic germ cells and the co-localization of MEIOB protein with RPA on chromosome axes. MEIOB localization in Dmc1-/- spermatocytes indicated that it accumulates on resected DNA. Homologous Meiob deletion in mice caused infertility in both sexes, due to a meiotic arrest at a zygotene/pachytene-like stage. DNA double strand break repair and homologous chromosome synapsis were impaired in Meiob-/- meiocytes. Interestingly MEIOB appeared to be dispensable for the initial loading of recombinases but was required to maintain a proper number of RAD51 and DMC1 foci beyond the zygotene stage. In light of these findings, we propose that RPA and this new single-strand DNA binding protein MEIOB, are essential to ensure the proper stabilization of recombinases which is required for successful homology search and meiotic recombination.
ISSN:1553-7390
1553-7404
DOI:10.1371/journal.pgen.1003784