Erysipelothrix rhusiopathiae Isolates Recovered from Fish, a Harbour Seal (Phoca vitulina) and the Marine Environment are Capable of Inducing Characteristic Cutaneous Lesions in Pigs

In order to determine the diversity and pathogenicity of Erysipelothrix spp. isolates recovered from marine fish, a harbour seal (Phoca vitulina) and the marine environment, 14 isolates were characterized by genotyping, serotyping, determination of the surface protective antigen (spa) gene type and...

Full description

Saved in:
Bibliographic Details
Published in:Journal of comparative pathology 2013-05, Vol.148 (4), p.365-372
Main Authors: Opriessnig, T., Shen, H.G., Bender, J.S., Boehm, J.R., Halbur, P.G.
Format: Article
Language:English
Subjects:
Animals
antigens
bioassays
Erysipelothrix
Erysipelothrix - genetics
Erysipelothrix - isolation & purification
Erysipelothrix - pathogenicity
Erysipelothrix Infections - immunology
Erysipelothrix Infections - pathology
Erysipelothrix Infections - transmission
Erysipelothrix rhusiopathiae
Erysipelothrix spp
fish
Fishes
genes
genotyping
harbour seal
Marine
marine environment
marine fish
Phoca
Phoca vitulina
pig
serotypes
Serotyping
Skin - immunology
Skin - pathology
Skin Diseases - immunology
Skin Diseases - pathology
Skin Diseases - veterinary
skin lesions
Swine
Swine Diseases - immunology
Swine Diseases - pathology
Swine Diseases - transmission
virulence
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In order to determine the diversity and pathogenicity of Erysipelothrix spp. isolates recovered from marine fish, a harbour seal (Phoca vitulina) and the marine environment, 14 isolates were characterized by genotyping, serotyping, determination of the surface protective antigen (spa) gene type and assessment of virulence in a pig bioassay. All 14 isolates were Erysipelothrix rhusiopathiae. Isolates were determined to be of serotypes 2 (n = 3), 3 (n = 1), 4 (n = 1), 12 (n = 1), 15 (n = 1) or 21 (n = 6), and one isolate cross-reacted with serotypes 5 and 21. The spa gene analysis determined that 64.3% (n = 9) were spaA and 35.7% (n = 5) were spaB1. In pigs, 10/14 isolates induced small plaques to diamond-shaped cutaneous lesions consistent with Erysipelothrix spp. infection. The results of this study indicate that the marine E. rhusiopathiae isolates have greater genetic and antigenic diversity than pig isolates and are capable of inducing classical skin lesions in pigs.
ISSN:0021-9975
1532-3129
DOI:10.1016/j.jcpa.2012.08.004