Urine Cell-Free DNA integrity as a marker for early bladder cancer diagnosis: Preliminary data

Abstract Objectives Urine cell-free (UCF) DNA has recently been proposed as a potential marker for early bladder cancer diagnosis. It is known that normal apoptotic cells produce highly fragmented DNA while cancer cells release longer DNA. Therefore, we verified the potential role of UCF DNA integri...

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Published in:Urologic oncology 2013-11, Vol.31 (8), p.1744-1750
Main Authors: Casadio, Valentina, B.S, Calistri, Daniele, Ph.D, Tebaldi, Michela, B.S, Bravaccini, Sara, B.S, Gunelli, Roberta, M.D, Martorana, Giuseppe, M.D, Bertaccini, Alessandro, M.D, Serra, Luigi, M.D, Scarpi, Emanuela, M.S, Amadori, Dino, M.D, Silvestrini, Rosella, Ph.D, Zoli, Wainer, Ph.D
Format: Article
Language:English
Subjects:
Aged
Biomarkers, Tumor - genetics
Biomarkers, Tumor - urine
DNA - genetics
DNA - urine
Early Detection of Cancer
Female
Humans
Male
Neoplasm Proteins - genetics
Proto-Oncogene Proteins c-myc - genetics
Real-Time Polymerase Chain Reaction
Receptor, ErbB-2 - genetics
Reference Values
ROC Curve
Spectrophotometry
Urinary Bladder Neoplasms - diagnosis
Urinary Bladder Neoplasms - genetics
Urinary Bladder Neoplasms - urine
Urology
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Summary:Abstract Objectives Urine cell-free (UCF) DNA has recently been proposed as a potential marker for early bladder cancer diagnosis. It is known that normal apoptotic cells produce highly fragmented DNA while cancer cells release longer DNA. Therefore, we verified the potential role of UCF DNA integrity in early bladder cancer diagnosis. Materials and methods UCF DNA was isolated from 51 bladder cancer patients, 46 symptomatic patients, and 32 healthy volunteers. To verify UCF DNA integrity, sequences longer than 250 bp, c-Myc , BCAS1 , and HER2 , were quantified by real time PCR. Results At the best cutoff value of 0.1 ng/μl, UCF DNA integrity analysis showed a sensitivity of 0.73 (95% CI 0.61–0.85), and a specificity of 0.84 (95% CI 0.71–0.97) in healthy individuals and 0.83 (95% CI 0.72–0.94) in symptomatic patients. Receiver operating characteristic (ROC) curve analysis revealed an area under the curve of 0.834 (95% CI 0.739–0.930) for healthy individuals and 0.796 (95% CI 0.707–0.885) for symptomatic patients. Conclusions These preliminary data suggest that UCF DNA integrity is a potentially good marker for early noninvasive diagnosis of bladder cancer. Its diagnostic performance does not seem to vary significantly, even in an “at risk” population of symptomatic individuals.
ISSN:1078-1439
1873-2496
DOI:10.1016/j.urolonc.2012.07.013