Transfer of resistance to the beet cyst nematode (Heterodera schachtii Schm.) into the Brassica napus L. gene pool through intergeneric somatic hybridization with Raphanus sativus L

An intergeneric somatic hybrid was obtained through PEG-induced protoplast fusion between Brassica napus L. (oil-seed rape, AACC, 2n = 38) and a beet cyst nematode resistant genotype of Raphanus sativus L. (fodder radish, RR, 2n = 18). The hybrid nature of the regenerated plant was confirmed by flow...

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Bibliographic Details
Published in:Theoretical and applied genetics 1992-04, Vol.83 (6-7), p.887-894
Main Authors: Lelivelt, C.L.C. (Centre for Plant Breeding and Reproduction Research, Wageningen (Netherlands)), Krens, F.A
Format: Article
Language:English
Subjects:
Agriculture
Agronomy
Biological and medical sciences
BRASSICA NAPUS
Brassica napus L
Classical genetics, quantitative genetics, hybrids
Fundamental and applied biological sciences. Psychology
GENETIC TRANSFORMATION
Genetics & Heredity
Genetics of eukaryotes. Biological and molecular evolution
HETERODERA SCHACHTII
HIBRIDO SOMATICO
Horticulture
HYBRIDE SOMATIQUE
Life Sciences & Biomedicine
Nematode resistance
PEST RESISTANCE
Plant Sciences
Pteridophyta, spermatophyta
RAPHANUS SATIVUS
Raphanus sativus L
RESISTANCE AUX ORGANISMES NUISIBLES
RESISTENCIA A LAS PLAGAS
Restriction fragment length polymorphism (RFLP)
Science & Technology
Somatic hybridization
SOMATIC HYBRIDS
TRANSFORMACION GENETICA
TRANSFORMATION GENETIQUE
Vegetals
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Summary:An intergeneric somatic hybrid was obtained through PEG-induced protoplast fusion between Brassica napus L. (oil-seed rape, AACC, 2n = 38) and a beet cyst nematode resistant genotype of Raphanus sativus L. (fodder radish, RR, 2n = 18). The hybrid nature of the regenerated plant was confirmed by flow cytometric analysis, RFLP-analysis, and chromosome counts. Southern blot analysis of total DNA using pPhcPS1 (rbc-L) as probe indicated that the somatic hybrid contains chloroplasts of B. napus. The mitochondrial genome of the somatic hybrid was studied more extensively using several probes and restriction enzymes. The results indicate inter- or intraspecific mitochondrial DNA recombination. Resistance to the beet cyst nematode (Heterodera schachtii Schm., BCN) was expressed in the hybrid at a high level.
ISSN:0040-5752
1432-2242
DOI:10.1007/BF00226712