A highly sensitive and wide-ranged electrochemical zinc(II) aptasensor fabricated on core–shell SiO2-Pt@meso-SiO2

In this work, bi-functional SiO2-Pt@meso-SiO2 core–shell nanoparticles were designed to prepare a highly sensitive and selective electrochemical zinc(II) aptasesnor. This core–shell structure boasts its SiO2 mesoporous shell and the inside Pt nanoparticles. SiO2 mesoporous shell can fix aptamer with...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2014-02, Vol.52, p.293-297
Main Authors: Li, Zhengxin, Liu, Meichuan, Fan, Lifang, Ke, Hongyang, Luo, Caifang, Zhao, Guohua
Format: Article
Language:English
Subjects:
Aptamer
Aptamers, Peptide - chemistry
Biological and medical sciences
Biosensing Techniques - methods
Biotechnology
Electrochemical aptasensor
Electrochemical Techniques - methods
Fundamental and applied biological sciences. Psychology
Humans
MCF-7 Cells
Platinum - chemistry
Silicon Dioxide - chemistry
SiO2
Zinc
Zinc - blood
Zinc - chemistry
Zinc - isolation & purification
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Summary:In this work, bi-functional SiO2-Pt@meso-SiO2 core–shell nanoparticles were designed to prepare a highly sensitive and selective electrochemical zinc(II) aptasesnor. This core–shell structure boasts its SiO2 mesoporous shell and the inside Pt nanoparticles. SiO2 mesoporous shell can fix aptamer without affecting its configuration and can admit electrolyte through the shell. SiO2 core inside can be the substrate of larger amount of Pt nanoparticles that improve the conductivity of the modified electrode dramatically. Due to the application of such a special bi-functional structure and the aptamer's strong combination capacity with Zn2+, Zn2+ is pre-enriched onto the electrode effectively and specifically, so that it can be determined sensitively and selectively. Results have shown that the zinc(II) aptasensor can be utilized at a wide linear working range from 100pM to 50μM and a low detection limit of 65pM, which makes it practical in both biological samples and environment monitoring. This method has been successfully applied in Zn2+ monitoring in human blood and disrupted human cells (MCF-7). •It is the first time that zinc aptamer is used to prepare zinc(II) aptasensor.•SiO2-Pt@meso SiO2 nanoparticles are utilized to fix aptamer and ensure conductivity.•Zinc(II) aptasensor has a very wide detection range from 100pM to 50μM.•Zinc(II) aptasensor is applied in zinc detection of ZnB and MCF-7.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2013.08.056