Long-term chilling of young tomato plants under low light and subsequent recovery. II. Chlorophyll fluorescence, carbon metabolism and activity of ribulose-1,5-bisphosphate carboxylase/oxygenase

To identify possible reasons for the persisting impairment of photosynthesis after long-term chilling, young tomato (Lycopersicon esculentum Mill.) plants were exposed to 6-10 degrees C for two weeks under low illumination during the daily light period (60-100 micromole quanta.m-2.s-1). The time cou...

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Bibliographic Details
Published in:Planta 1992, Vol.186 (2), p.179-187
Main Authors: Bruggemann, W. (Heinrich-Heine-Universitat, Dusseldorf, FRG), Kooij, T.A.W. van der, Hasselt, P.R. van
Format: Article
Language:English
Subjects:
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
Biological and medical sciences
CHLOROPHYLLE
Chlorophylls
CLOROFILAS
Cooling
ENFRIAMIENTO
Fluorescence
FOTOSINTESIS
Fundamental and applied biological sciences. Psychology
Leaves
LIASAS
Low temperature
LUMIERE
LUZ
LYASE
LYCOPERSICON ESCULENTUM
Metabolism
METABOLISME DES GLUCIDES
METABOLISMO DE CARBOHIDRATOS
Phosphates
PHOTOSYNTHESE
Photosynthesis
Photosynthesis, respiration. Anabolism, catabolism
Plant physiology and development
Plants
PROPIEDADES OPTICAS
PROPRIETE OPTIQUE
REFROIDISSEMENT
Starches
Sugars
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Summary:To identify possible reasons for the persisting impairment of photosynthesis after long-term chilling, young tomato (Lycopersicon esculentum Mill.) plants were exposed to 6-10 degrees C for two weeks under low illumination during the daily light period (60-100 micromole quanta.m-2.s-1). The time courses of leaf carbohydrate contents, phosphorylated intermediates and chlorophyll-fluorescence parameters were followed. While starch formation was impaired during chilling at 6 degrees C, soluble sugar contents increased from the first day onwards and reached up to eightfold the values found in unchilled plants within two weeks. At 8 and 10 degrees C, a less drastic increase in soluble-carbohydrate contents was observed. During chilling, glucose-6-phosphate and fructose-6-phosphate accumulated up to 16 mM (assuming they are restricted to the cytoplasm). At the same time, non-photochemical quenching of chlorophyll fluorescence had increased and did not return to control values during the first week of recovery. The 3-phosphoglyceric acid/triose phosphate ratio remained nearly unaffected by the chilling treatment, indicating that the assimilatory power of the plants was still high even at the low temperatures. As a consequence of the chilling treatment, ribulose-1,5-biphosphate carboxylase/oxygenase (Rubisco) activity in the chilled leaves was irreversibly decreased. It is suggested that, in addition to a possible (orthophosphate-mediated) feedback inhibition by internal sugar accumulation, the low activity of Rubisco can play a significant role in the strong decrease of photosynthetic capacity during long-term chilling in tomato
ISSN:0032-0935
1432-2048
DOI:10.1007/BF00196247