Proteomic Analysis of Leaves and Roots of Common Wheat (Triticum aestivum L.) under Copper-Stress Conditions

Proteomic studies were performed to identify the protein species involved in copper (Cu) stress responses in common wheat. Two-week-old wheat seedlings were exposed to 100 μM CuSO4 treatment for 3 days. Growth of shoots and roots was markedly inhibited and lipid peroxidation was greatly increased. C...

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Bibliographic Details
Published in:Journal of proteome research 2013-11, Vol.12 (11), p.4846-4861
Main Authors: Li, Gezi, Peng, Xiaoqi, Xuan, Hongmei, Wei, Liting, Yang, Yingying, Guo, Tiancai, Kang, Guozhang
Format: Article
Language:English
Subjects:
Analysis of Variance
Copper Sulfate - pharmacokinetics
Copper Sulfate - toxicity
Cyclopentanes - pharmacology
DNA Primers - genetics
Electrophoresis, Gel, Two-Dimensional
Gene Expression Regulation, Plant - drug effects
Gene Expression Regulation, Plant - genetics
Glutathione Transferase - metabolism
Image Processing, Computer-Assisted
Lipid Peroxidation - drug effects
Oxylipins - pharmacology
Plant Leaves - genetics
Plant Leaves - metabolism
Plant Proteins - genetics
Plant Proteins - metabolism
Plant Roots - genetics
Plant Roots - metabolism
Proteomics
Stress, Physiological - genetics
Tandem Mass Spectrometry
Triticum - genetics
Triticum - metabolism
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Summary:Proteomic studies were performed to identify the protein species involved in copper (Cu) stress responses in common wheat. Two-week-old wheat seedlings were exposed to 100 μM CuSO4 treatment for 3 days. Growth of shoots and roots was markedly inhibited and lipid peroxidation was greatly increased. Cu was readily absorbed by wheat seedlings, with greater Cu contents in roots than in leaves. Using 2-DE method, 98 protein spots showed significantly enhanced or reduced abundance, of which 93 were successfully identified. Of these identified protein species, 49 and 44 were found in roots and leaves, respectively. Abundance of most of identified protein species, which function in signal transduction, stress defense, and energy production, was significantly enhanced, while that of many protein species involved in carbohydrate metabolism, protein metabolism, and photosynthesis was severely reduced. The Cu-responsive protein interaction network revealed 36 key proteins, most of which may be regulated by abscisic acid (ABA), ethylene, jasmonic acid (JA), and so on. Exogenous JA application showed a protective effect against Cu stress and significantly increased transcripts of the glutathione S-transferase (GST) gene. This study provides insight into the molecular mechanisms of Cu responses in higher plants.
ISSN:1535-3893
1535-3907
DOI:10.1021/pr4008283