PCR-based fingerprinting using AFLPs as a tool for studying genetic relationships in Lactuca spp

AFLP markers were evaluated for determining the phylogenetic relationships Lactuca spp. Genetic distances based on AFLP data were estimated for 44 morphologically diverse lines of cultivated L. sativa and 13 accessions of the wild species L. serriola, L. saligna, L. virosa, L. perennis, and L. indic...

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Bibliographic Details
Published in:Theoretical and applied genetics 1996-12, Vol.93 (8), p.1202-1210
Main Authors: Hill, M, Witsenboer, H, Zabeau, M, Vos, P, Kesseli, R, Michelmore, R. (Massachusetts Univ., Boston (USA). Dept. of Biology)
Format: Article
Language:English
Subjects:
Biological and medical sciences
Classical genetics, quantitative genetics, hybrids
DISTANCE GENETIQUE
DISTANCIA GENETICA
FILOGENIA
Fundamental and applied biological sciences. Psychology
GENETIC DISTANCE
GENETIC MARKERS
Genetics of eukaryotes. Biological and molecular evolution
LACTUCA INDICA
LACTUCA SERRIOLA
LACTUCA VIROSA
MARCADORES GENETICOS
MARQUEUR GENETIQUE
PHYLOGENIE
PHYLOGENY
Pteridophyta, spermatophyta
Vegetals
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Summary:AFLP markers were evaluated for determining the phylogenetic relationships Lactuca spp. Genetic distances based on AFLP data were estimated for 44 morphologically diverse lines of cultivated L. sativa and 13 accessions of the wild species L. serriola, L. saligna, L. virosa, L. perennis, and L. indica. The same genotypes were analyzed as in a previous study that had utilized RFLP markers. The phenetic tree based on AFLP data was consistent with known taxonomic relationships and similar to a tree developed with RFLP data. The genetic distance matrices derived from AFLP and RFLP data were compared using least squares regression analysis and, for the cultivar data, by principal component analysis. There was also a positive linear relationship between distance estimates based on AFLP data and kinship coefficients calculated from pedigree data. AFLPs represent reliable PCR-based markers for studies of genetic relationships at a variety of taxonomic levels.
ISSN:0040-5752
1432-2242
DOI:10.1007/bf00223451