Rapid high-yield purification of canine intestinal motilin and its complete sequence determination

Canine niotilin has been purified from small amounts of canine intestine in a form suitable for microsequence analysis. The sequence determined is: Phe-Val-Pro-Ile-Phe-Thr-His-Ser-Glu-Leu-Gln-Lys-Ile-Arg-Glu-Lys-Glu-Arg-Asn-Lys-Ile-Arg-Asn-Lys-Gly-Gln. Canine motilin differs from porcine motilin at...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Chromatography 1985-01, Vol.321 (2), p.421-432
Main Authors: Reeve, J.R., Ho, F.J., Walsh, J.H., Ben-Avram, C.M., Shively, J.E.
Format: Article
Language:English
Subjects:
amino acid sequence
Aminoacids, peptides. Hormones. Neuropeptides
Analytical, structural and metabolic biochemistry
Biological and medical sciences
dogs
Fundamental and applied biological sciences. Psychology
intestinal motility
motilin
Proteins
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Canine niotilin has been purified from small amounts of canine intestine in a form suitable for microsequence analysis. The sequence determined is: Phe-Val-Pro-Ile-Phe-Thr-His-Ser-Glu-Leu-Gln-Lys-Ile-Arg-Glu-Lys-Glu-Arg-Asn-Lys-Ile-Arg-Asn-Lys-Gly-Gln. Canine motilin differs from porcine motilin at five positions. The rapid, high-yield (24% overall yield) microisolation techniques used for canine motilin should be suitable for the isolation of other basic peptides found in low levels in tissue that is available only in limited amounts. These methods should make the isolation and sequence determination of human brain and gut peptides more readily achievable.
ISSN:0021-9673
DOI:10.1016/S0021-9673(01)90460-1