Anti adipogenic activity of Aegle marmelos Correa

In continuation of evaluating the anti-obesity effect of Aegle marmelos, we have screened the n-hexane, dichloro methane (DCM), ethyl acetate (EtOAc) and methanol (MeOH) extracts of the leaves at the concentration of 25, 50, 75 and 100μg/ml for adipogenesis inhibition in the adipocytes. Nile red sta...

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Bibliographic Details
Published in:Phytomedicine (Stuttgart) 2013-11, Vol.20 (14), p.1267-1271
Main Authors: Karmase, Aniket, Jagtap, Sneha, Bhutani, Kamlesh K.
Format: Article
Language:English
Subjects:
3T3-L1 Cells
Adipogenesis
Adipogenesis - drug effects
Adipose Tissue - cytology
Adipose Tissue - drug effects
Adipose Tissue - metabolism
Aegle - chemistry
Aegle marmelos
Amides - isolation & purification
Amides - pharmacology
Animals
Anti-obesity
Anti-Obesity Agents - pharmacology
Ethyl ether aegeline
Halfordinol
Lipid Metabolism - drug effects
Mice
Nile red
Obesity
Obesity - prevention & control
Phytochemistry
Plant Extracts - chemistry
Plant Extracts - pharmacology
Prevention
Umbelliferones - isolation & purification
Umbelliferones - pharmacology
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Summary:In continuation of evaluating the anti-obesity effect of Aegle marmelos, we have screened the n-hexane, dichloro methane (DCM), ethyl acetate (EtOAc) and methanol (MeOH) extracts of the leaves at the concentration of 25, 50, 75 and 100μg/ml for adipogenesis inhibition in the adipocytes. Nile red staining with the help of fluorometry was used as indicator of the antiobesity activity. The most active DCM extract showed the 33.98±3.55% lipid content at 100μg/ml and was selected for the further isolation. 14 compounds were isolated from DCM extract of A. marmelos leaves. The compounds were screened for the adipogenesis inhibition at 50 and 100μM concentrations. Out of the 14 compounds, halfordinol, ethyl ether aegeline and esculetin were showing 10.04±0.52, 16.29±0.85 and 25.09±1.31% lipid content respectively at 100μM. We hereby report the adipogenesis inhibition by A. marmelos as one of the pathway for its antiobesity effect.
ISSN:0944-7113
1618-095X
DOI:10.1016/j.phymed.2013.07.011