A Novel Hydrogen Peroxide Biosensor Based on Adsorption of Horseradish Peroxidase onto a Nanobiomaterial Composite Modified Glassy Carbon Electrode

In this work, a novel hydrogen peroxide biosensor derived from maize tassel (MT) and multiwalled carbon nanotube (MWCNT) composite was used to adsorb horseradish peroxidase (HRP) onto the surface of a glassy carbon electrode through electrostatic interactions. The morphology and structure of the pro...

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Bibliographic Details
Published in:Electroanalysis (New York, N.Y.) N.Y.), 2013-08, Vol.25 (8), p.1946-1954
Main Authors: Moyo, Mambo, Okonkwo, Jonathan O., Agyei, Nana M.
Format: Article
Language:English
Subjects:
Adsorption
Glassy carbon electrode
Horseradish peroxidase
Hydrogen peroxide
Maize tassel
Multiwalled carbon nanotube
Zea mays
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Summary:In this work, a novel hydrogen peroxide biosensor derived from maize tassel (MT) and multiwalled carbon nanotube (MWCNT) composite was used to adsorb horseradish peroxidase (HRP) onto the surface of a glassy carbon electrode through electrostatic interactions. The morphology and structure of the products were characterized by SEM, FTIR and UV‐visible spectroscopy. The electrochemical and electrocatalytic performance of the HRP/MT‐MWCNT/GCE was studied using voltammetric and amperometric methods. The amperometric response of the biosensor varied linearly with concentration of H2O2 from 9 µM to 1 mM with detection limit of 4.0 µM (S/N=3). Furthermore, the biosensor exhibited good reproducibility and stability.
ISSN:1040-0397
1521-4109
DOI:10.1002/elan.201300165