Phorbol Ester or Epidermal Growth-factor-induced MUC5AC Mucin Gene Expression and Production from Airway Epithelial Cells are Inhibited by Apigenin and Wogonin

In this study, we investigated whether apigenin and wogonin affect MUC5AC mucin production and gene expression induced by phorbol ester (phorbol 12‐myristate 13‐acetate, PMA) or epidermal growth factor (EGF) from human airway epithelial cells. Confluent NCI‐H292 cells were pretreated with each agent...

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Bibliographic Details
Published in:Phytotherapy research 2012-12, Vol.26 (12), p.1784-1788
Main Authors: Kim, Ju-Ock, Sikder, Md. Asaduzzaman, Lee, Hyun Jae, Rahman, Mustafizur, Kim, Jang-Hyun, Chang, Gyu Tae, Lee, Choong Jae
Format: Article
Language:English
Subjects:
airway mucin
apigenin
Apigenin - pharmacology
Biological and medical sciences
Cell Line
Enzyme-linked immunosorbent assay
Epidermal Growth Factor - pharmacology
Epithelial Cells - drug effects
Epithelial Cells - metabolism
Flavanones - pharmacology
General pharmacology
Humans
Medical sciences
MUC5AC
Mucin 5AC - genetics
Mucin 5AC - metabolism
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Phorbol Esters - pharmacology
wogonin
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Summary:In this study, we investigated whether apigenin and wogonin affect MUC5AC mucin production and gene expression induced by phorbol ester (phorbol 12‐myristate 13‐acetate, PMA) or epidermal growth factor (EGF) from human airway epithelial cells. Confluent NCI‐H292 cells were pretreated with each agent for 30 min and then stimulated with PMA or EGF for 24 h, respectively. MUC5AC mucin gene expression and mucin protein production were measured by reverse transcription polymerase chain reaction (RT‐PCR) and enzyme linked immunosorbent assay (ELISA). The results were as follows: (i) apigenin and wogonin were found to inhibit the production of MUC5AC mucin protein induced by PMA or EGF; (ii) both compounds also inhibited the expression of MUC5AC mucin gene induced by PMA or EGF. These results suggest that apigenin and wogonin can inhibit mucin gene expression and production of mucin protein, by directly acting on airway epithelial cells. Copyright © 2012 John Wiley & Sons, Ltd.
ISSN:0951-418X
1099-1573
DOI:10.1002/ptr.4650