Genetic diversity of populations and clones of Rhopilema esculentum in China based on AFLP analysis

Amplified fragment length polymorphisms （AFLP） markers were developed to assess the genetic variation of populations and clones of Rhopilema esculentum Kishinouye （Scyphozoa, Rhizostomatidae）. One hundred and seventy-nine loci from 56 individuals of two hatchery populations and two wild populations...

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Bibliographic Details
Published in:Chinese journal of oceanology and limnology 2013-03, Vol.31 (2), p.391-397
Main Author: 乔洪金 刘相全 张锡佳 姜海滨 王际英 张利民
Format: Article
Language:English
Subjects:
AFLP分析
Amplified fragment length polymorphism
Clones
Cluster analysis
Differentiation
Earth and Environmental Science
Earth Sciences
Genetic diversity
Genetic markers
Genetic variation
Germplasm
germplasm conservation
Hatcheries
Heterozygosity
Invertebrates
loci
Marine
Marine biology
Marine invertebrates
Mathematics
Oceanography
Polymorphism
Population genetics
Populations
Rhizostomatidae
Rhopilema esculentum
Scyphozoa
sowing
中国
克隆
养殖群体
扩增片段长度多态性
海蜇
遗传多样性
野生种群
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Summary:Amplified fragment length polymorphisms （AFLP） markers were developed to assess the genetic variation of populations and clones of Rhopilema esculentum Kishinouye （Scyphozoa, Rhizostomatidae）. One hundred and seventy-nine loci from 56 individuals of two hatchery populations and two wild populations were genotyped with five primer combinations. The polymorphic ratio, Shannon＇s diversity index and average heterozygosity were 70.3%, 0.346 and 0.228 for the white hatchery population, 74.3%, 0.313, and 0.201 for the red hatchery population, 79.3%, 0.349, and 0.224 for the Jiangsu wild population, and 74.9%, 0.328 and 0.210 for the Penglai wild population, respectively. Thus, all populations had a relatively high level of genetic diversity. A specific band was identified that could separate the white from the red hatchery population. There was 84.85% genetic differentiation within populations. Individual cluster analysis using unweighted pair-group method with arithmetic mean （UPGMA） suggested that hatchery populations and wild populations could be divided. For the hatchery populations, the white and red populations clustered separately; however, for the wild populations, Penglai and Jiangsu populations clustered together. The genetic diversity at the clone level was also determined. Our data suggest that there are relatively high genetic diversities within populations but low genetic differentiation between populations, which may be related to the long-term use of germplasm resources from Jiangsu Province for artificial seeding and releasing. These findings will benefit the artificial seeding and conservation of the germplasm resources.
ISSN:0254-4059
2096-5508
1993-5005
2523-3521
DOI:10.1007/s00343-013-2158-3