Digital holographic microtomography for high-resolution refractive index mapping of live cells

Quantification of three‐dimensional (3D) refractive index (RI) with sub‐cellular resolution is achieved by digital holographic microtomography (DHμT) using quantitative phase images measured at multiple illumination angles. The DHμT system achieves sensitive and fast phase measurements based on iter...

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Bibliographic Details
Published in:Journal of biophotonics 2013-05, Vol.6 (5), p.416-424
Main Authors: Su, Jing-Wei, Hsu, Wei-Chen, Chou, Cheng-Ying, Chang, Chen-Hao, Sung, Kung-Bin
Format: Article
Language:English
Subjects:
Cell Line, Tumor
Cell Survival
digital holographic microscopy
Holography - methods
Humans
image reconstruction
Imaging, Three-Dimensional - methods
interferometry
Intracellular Space - metabolism
refractive index
Studies
three-dimensional imaging
X-Ray Microtomography - methods
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Summary:Quantification of three‐dimensional (3D) refractive index (RI) with sub‐cellular resolution is achieved by digital holographic microtomography (DHμT) using quantitative phase images measured at multiple illumination angles. The DHμT system achieves sensitive and fast phase measurements based on iterative phase extraction algorithm and asynchronous phase shifting interferometry without any phase monitoring or active control mechanism. A reconstruction algorithm, optical diffraction tomography with projection on convex sets and total variation minimization, is implemented to substantially reduce the number of angular scattered fields needed for reconstruction without sacrificing the accuracy and quality of the reconstructed 3D RI distribution. Tomogram of a living CA9‐22 cell is presented to demonstrate the performance of the method. Further, a statistical analysis of the average RI of the nucleoli, the nucleus excluding the nucleoli and the cytoplasm of twenty CA9‐22 cells is performed. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
ISSN:1864-063X
1864-0648
DOI:10.1002/jbio.201200022