Involvement of Inducible Costimulator Ligand (ICOSL) Expression in Thyroid Tissue in Hyperthyroidism of Graves’ Disease Patients

Background The role of costimulatory molecules expressed on lymphocytes and thyrocytes in hyperthyroidism has attracted increasing attention and research has shown a close correlation between variant expression of these molecules on lymphocytes and thyrocytes and the development of GD. Meterials and...

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Bibliographic Details
Published in:Journal of clinical immunology 2012-12, Vol.32 (6), p.1253-1261
Main Authors: Wang, Fengming, Yan, Tao, Chen, Lujun, Chen, Xuemin, Liu, Tong, Shen, Shuang, Li, Ting, Gao, Li, Wang, Ting, Sun, Jing, Liu, Cuiping, Wu, Haorong, Zhang, Xueguang, Chen, Lei
Format: Article
Language:English
Subjects:
Adult
Animals
Antibodies - pharmacology
Biomedical and Life Sciences
Biomedicine
Cell Line
Cell Movement - drug effects
Cell Proliferation - drug effects
Female
Gene Expression - drug effects
Goiter, Endemic - genetics
Goiter, Endemic - immunology
Goiter, Endemic - metabolism
Goiter, Endemic - pathology
Graves Disease - genetics
Graves Disease - immunology
Graves Disease - metabolism
Graves Disease - pathology
Hashimoto Disease - genetics
Hashimoto Disease - immunology
Hashimoto Disease - metabolism
Hashimoto Disease - pathology
Humans
Immunology
Inducible T-Cell Co-Stimulator Ligand - antagonists & inhibitors
Inducible T-Cell Co-Stimulator Ligand - genetics
Inducible T-Cell Co-Stimulator Ligand - metabolism
Infectious Diseases
Interferon-gamma - pharmacology
Interleukin-6 - pharmacology
Internal Medicine
Lymphocytes - drug effects
Lymphocytes - immunology
Lymphocytes - pathology
Male
Medical Microbiology
Mice
Middle Aged
Primary Cell Culture
Signal Transduction - drug effects
Thyroid Gland - immunology
Thyroid Gland - metabolism
Thyroid Gland - pathology
Transfection
Tumor Necrosis Factor-alpha - pharmacology
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Summary:Background The role of costimulatory molecules expressed on lymphocytes and thyrocytes in hyperthyroidism has attracted increasing attention and research has shown a close correlation between variant expression of these molecules on lymphocytes and thyrocytes and the development of GD. Meterials and Methods Thyroid tissues were collected from GD patients during surgery and from Hashimoto disease (HT) and non-toxic goiter (NTG) patients as controls. ICOSL expression on infiltrated B cells and TFC was detected by flow cytometry (FCM), reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry (IHC). Variation in ICOSL expression on TFC in primary cultures was analyzed in the absence or presence of cytokines using FCM assays. The role of ICOS-ICOSL signaling in proliferation, thyroid hormone production and thyroglobulin (Tg) release was investigated in primary TFC cultures using ICOS gene transfected L929 cells (ICOS-L929 cells) and the blocking ICOSL antibody (11 C4) in MTT assays and radioimmunoassays. Results and Discussion ICOSL expression on infiltrated B cells and TFC was detected in GD patient tissue. However, ICOSL expression was only detected on infiltrated B cells in control HT and NTG patient tissue. ICOSL expression on TFC was induced in vitro by the proinflammatory cytokines IFN-γ, IL-6 and TNF-α. Compared with mock transfected L929 (mock-L929) control cells, ICOS-L929 cells promoted significant proliferation of primary cultured TFC, with increased thyroid hormone and Tg production (all P  
ISSN:0271-9142
1573-2592
DOI:10.1007/s10875-012-9711-2