Development of a Selective Enzyme-Linked Immunosorbent Assay for 1-Naphthol-the Major Metabolite of Carbaryl (1-Naphthyl N-Methylcarbamate)

A selective enzyme-linked immunosorbent assay for 1-naphthol was developed. Eight rabbits were immunized with either [(5-hydrosy-2-naphthylenyl)oxylacetic acid (2b)-BSA, [(5-hydroxy-1-naphthylenyl)osy]acetic acid (4)-BSA, or 5-hydrosy-2-naphthoic acid (7)-KLH, and all sera were screened against 14 d...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 1994-04, Vol.42 (4), p.934-943
Main Authors: Kramer, Petra M, Marco, Maria-Pilar, Hammock, Bruce D
Format: Article
Language:English
Subjects:
1-naphthol
Animal, plant and microbial ecology
Applied ecology
AUXINAS
AUXINE
Biological and medical sciences
CARBARIL
CARBARYL
chemical analysis
Chemical control
Control
Ecotoxicology, biological effects of pollution
ELISA
enzyme-linked immunosorbent assay
Fundamental and applied biological sciences. Psychology
Invertebrates
Medical sciences
METABOLITE
metabolites
METABOLITOS
Pesticides, fertilizers and other agrochemicals toxicology
Phytopathology. Animal pests. Plant and forest protection
Protozoa. Invertebrates
TECHNIQUE ANALYTIQUE
TECNICAS ANALITICAS
Terrestrial environment, soil, air
TEST ELISA
Toxicology
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Summary:A selective enzyme-linked immunosorbent assay for 1-naphthol was developed. Eight rabbits were immunized with either [(5-hydrosy-2-naphthylenyl)oxylacetic acid (2b)-BSA, [(5-hydroxy-1-naphthylenyl)osy]acetic acid (4)-BSA, or 5-hydrosy-2-naphthoic acid (7)-KLH, and all sera were screened against 14 different coating antigens. From this screening procedure, five assays could be developed, using two 7-KLH antisera, 3907 and 3909. The assay with the least interference and the best reliability (7-KLH antiserum 3907, 1:9000, and coating antigen 2b-BSA, 1.5 micrograms/mL) was further optimized and characterized. It showed an I50 value of about 72 micrograms/L, with a range of detection from 10 to 1000 micrograms/L. There was no cross-reactivity with carbaryl, which makes this assay very suitable for the selective detection of 1-naphthol, for esample, in undiluted human urine and in soil extract. The assay was tested in organic solvents and showed a tolerance of at least 10% solvent (acetone, acetonitrile, methanol).
ISSN:0021-8561
1520-5118
DOI:10.1021/jf00040a019