Type II phosphatidylinositol 4-kinase [beta] is an integral signaling component of early T cell activation mechanisms

The early signaling events in T cell activation through CD3 receptor include a rapid change in intra cellular free calcium concentration and reorganization of actin cytoskeleton. Phosphatidylinositol 4-kinases (PtdIns 4-kinases) are implicated as key components in these early signaling events. The r...

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Bibliographic Details
Published in:Biochimie 2013-08, Vol.95 (8), p.1560-1566
Main Authors: Sinha, Ranjeet K, Bojjireddy, Naveen, Kulkarni, Dakshayini, Ratheesh, Aparna, Chiplunkar, S V, Gude, Rajiv, Subrahmanyam, Gosukonda
Format: Article
Language:English
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Summary:The early signaling events in T cell activation through CD3 receptor include a rapid change in intra cellular free calcium concentration and reorganization of actin cytoskeleton. Phosphatidylinositol 4-kinases (PtdIns 4-kinases) are implicated as key components in these early signaling events. The role of type II PtdIns 4-kinase [beta] in CD3 receptor signaling was investigated with the help of short hairpin RNA sequences. Cross-linking of CD3 receptors on Jurkat T Cells with monoclonal antibodies showed an early increase in type II PtdIns 4-kinase activity and co-localization of type II PtdIns 4-kinase [beta] with CD3 IPG. Transfection of Jurkat T Cells with shRNAs inhibited CD3 receptor mediated type II PtdIns 4-kinase activation with a concomitant reduction in intra cellular calcium release, suggesting a role for type II PtdIns 4-kinase [beta] in CD3 receptor signal transduction. Knock-down of type II PtdIns 4-kinase [beta] with shRNAs also correlated with a decrease in PtdIns 4-kinase activity in cytoskeleton fractions and reduced adhesion to matrigel surfaces. These results indicate that type II PtdIns 4-kinase [beta] is a key component in early T cell activation signaling cascades.
ISSN:0300-9084