Some characteristics of IL-5-producing T cells in mouse liver induced by Schistosoma japonicum infection

Schistosome infection could cause significant liver damage in animal; Th2 cells play an important role in the progress of this disease. In our study, C57BL/6 mice were infected by Schistosoma japonicum and lymphocytes were isolated from the liver to detect some characteristics of interleukin-5 (IL-5...

Full description

Saved in:
Bibliographic Details
Published in:Parasitology research (1987) 2013-05, Vol.112 (5), p.1945-1951
Main Authors: Xie, Hongyan, Chen, Dianhui, Luo, Xueping, Gao, Zhiyan, Fang, Huilong, Huang, Jun
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Biomedicine
Health aspects
Immunology
Interferon-gamma - biosynthesis
Interleukin-17 - metabolism
Interleukin-4 - biosynthesis
Interleukin-5
Interleukin-5 - biosynthesis
Liver
Liver - immunology
Liver - parasitology
Medical Microbiology
Mice
Mice, Inbred C57BL
Microbiology
Original Paper
Physiological aspects
Schistosoma
Schistosoma japonicum
Schistosoma japonicum - immunology
Schistosoma japonicum - pathogenicity
Schistosomiasis japonica - immunology
Schistosomiasis japonica - parasitology
T cells
T-Lymphocytes - cytology
T-Lymphocytes - immunology
T-Lymphocytes - metabolism
Th1 Cells - cytology
Th1 Cells - immunology
Th1 Cells - metabolism
Th2 Cells - cytology
Th2 Cells - immunology
Th2 Cells - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Schistosome infection could cause significant liver damage in animal; Th2 cells play an important role in the progress of this disease. In our study, C57BL/6 mice were infected by Schistosoma japonicum and lymphocytes were isolated from the liver to detect some characteristics of interleukin-5 (IL-5)-producing T cells by different methods. The results revealed that S . japonicum infection could induce a large amount of IL-5 in mouse liver T cells by the means of fluorescent bead immunoassay and RT-PCR. Although, mouse liver contained many T cell subsets, such as Th cells, Tc cells, NKT cells, and γδ T cells. Fluorescence activated cell sorting results indicated that Th cells were the main source of IL-5 in the T cell population after phorbol 12-myristate 13-acetate and ionomycin stimulation. Moreover, the percentage of IL-5-producing Th cells continued to increase from 4 to 8 weeks after S . japonicum infection, which differed from the changes of IFN-γ + Th1 cells, IL-4 + Th2 cells, and IL-17A + Th17 cells during S . japonicum infection. Additionally, cytokines co-expression results demonstrated that 36.2 % of IL-5 + Th cells could express IL-4, and 10 % of it could produce IFN-γ or IL-17A. Collectively, these findings implied that IL-5-producing Th cells posses some properties which differ from other cytokines secreting Th cells.
ISSN:0932-0113
1432-1955
DOI:10.1007/s00436-013-3350-2